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Effects of Deletions in <i>Pichia pastoris RTG</i>Genes on Phenotype and AOX1 Expression

Effects of Deletions in <i>Pichia pastoris RTG</i>Genes on Phenotype and AOX1 Expression
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摘要 Methylotrophic yeast Pichia pastoris is an object of modern biotechnology. Decisive understanding of gene regulation mechanisms is essential for successful protein production. In this study, we investigated the effect of deletions in P. pastoris genes encoding proteins, homologous to S. serevisiae Rtg1p, Rtg 2p, Msn2p and Msn4p. It was shown, that deletion in PpRTG1 gene results in inability of P. pastoris to grow on medium with methanol as a carbon source and ammonium sulfate as a source of nitrogen. We also demonstrate that deletions in PpRTG1 and PpRTG2 decrease activity of AOX1 promoter. Methylotrophic yeast Pichia pastoris is an object of modern biotechnology. Decisive understanding of gene regulation mechanisms is essential for successful protein production. In this study, we investigated the effect of deletions in P. pastoris genes encoding proteins, homologous to S. serevisiae Rtg1p, Rtg 2p, Msn2p and Msn4p. It was shown, that deletion in PpRTG1 gene results in inability of P. pastoris to grow on medium with methanol as a carbon source and ammonium sulfate as a source of nitrogen. We also demonstrate that deletions in PpRTG1 and PpRTG2 decrease activity of AOX1 promoter.
出处 《Advances in Microbiology》 2018年第5期439-450,共12页 微生物学(英文)
关键词 PICHIA PASTORIS AOX1 Tor-Kinase Retrograde Regulation Pathway Pichia pastoris AOX1 Tor-Kinase Retrograde Regulation Pathway
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