摘要
Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC<sub>50</sub> = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC<sub>50</sub> = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.
Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC<sub>50</sub> = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC<sub>50</sub> = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.
作者
Wendkouni Leila Marie Esther Belem-Kabré
Vincent Ouédraogo
Bagora Bayala
Alimata Bancé
Estelle Ouédraogo
Boubacar Yaro
Lazare Belemnaba
Moussa Compaoré
Martin Kiendrébeogo
Noufou Ouédraogo
Wendkouni Leila Marie Esther Belem-Kabré;Vincent Ouédraogo;Bagora Bayala;Alimata Bancé;Estelle Ouédraogo;Boubacar Yaro;Lazare Belemnaba;Moussa Compaoré;Martin Kiendrébeogo;Noufou Ouédraogo(Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso;Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso;Laboratory of Molecular Biology and Genetics (LABIOGENE), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso;International Research Laboratory: Environnement, Santé, Sociétés (IRL 3189, ESS) CNRST/CNRS/UCAD/UGB/USTTB, Ouagadougou, Burkina Faso)
