摘要
Purpose: Recently, Candida haemulonii complex (Candida haemulonii, Candida duobushaemulonii and Candida haemulonii var. vulnera) and two genetically close species (Candida pseudohaemulonii and Candida auris) have emerged as an opportunistic fungal pathogen associated with various infectious diseases of humans, and most of those isolates have displayed antifungal resistance. Because it is difficult to differentiate these microorganisms by a current technique, unfortunately, it is important to establish a method for identifying them accurately. The purpose of the present study was to design species-specific primers in order to identify and detect C. auris, C. pseudohaemulonii, and C. haemulonii complex, i.e. , C. haemulonii, C. duobushaemulonii and C. haemulonii var. vulnera using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 26S rRNA, 18S rRNA, and RPB1 genes and ITS region of five Candida species. Results: The multiplex PCR method developed in this study was able to distinguish five Candida species clearly. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction.
Purpose: Recently, Candida haemulonii complex (Candida haemulonii, Candida duobushaemulonii and Candida haemulonii var. vulnera) and two genetically close species (Candida pseudohaemulonii and Candida auris) have emerged as an opportunistic fungal pathogen associated with various infectious diseases of humans, and most of those isolates have displayed antifungal resistance. Because it is difficult to differentiate these microorganisms by a current technique, unfortunately, it is important to establish a method for identifying them accurately. The purpose of the present study was to design species-specific primers in order to identify and detect C. auris, C. pseudohaemulonii, and C. haemulonii complex, i.e. , C. haemulonii, C. duobushaemulonii and C. haemulonii var. vulnera using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 26S rRNA, 18S rRNA, and RPB1 genes and ITS region of five Candida species. Results: The multiplex PCR method developed in this study was able to distinguish five Candida species clearly. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction.
作者
Mana Fuchigami
Osamu Tsuzukibashi
Akira Fukatsu
Koji Umezawa
Sachiyo Hayashi
Yuji Takahashi
Hiroshi Yamamoto
Chiaki Komine
Mio Hagiwara-Hamano
Yukiko Iizuka
Satoshi Uchibori
Masanobu Wakami
Hiroshi Murakami
Taira Kobayashi
Masahiko Fukumoto
Mana Fuchigami;Osamu Tsuzukibashi;Akira Fukatsu;Koji Umezawa;Sachiyo Hayashi;Yuji Takahashi;Hiroshi Yamamoto;Chiaki Komine;Mio Hagiwara-Hamano;Yukiko Iizuka;Satoshi Uchibori;Masanobu Wakami;Hiroshi Murakami;Taira Kobayashi;Masahiko Fukumoto(Department of Laboratory Medicine for Dentistry for the Compromised Patient, School of Dentistry at Matsudo, Nihon University, Chiba, Japan;Department of Special Needs Dentistry, School of Dentistry at Matsudo, Nihon University, Chiba, Japan;Department of Oral Implantology, School of Dentistry at Matsudo, Nihon University, Chiba, Japan;Department of Oral Surgery, School of Dentistry at Matsudo, Nihon University, Chiba, Japan;Department of Fixed Prosthodontics, School of Dentistry at Matsudo, Nihon University, Chiba, Japan)
