摘要
This study aimed to investigate the tissue culture and rapid propagation techniques of Orostachys fimbriata, a medicinal and ornamental herbaceous perennial herb belonging to the family Crassulaceae. The leaves of O. fimbriata were used as explants to investigate the effects of plant growth regulators such as thidiazuron (TDZ), 6-benzylamino purine (6-BA) and 1-naphthaleneacetic acid (NAA), on the induction of callus, differentiation of adventitious buds and rooting of shoots. Our results showed that optimum callus induction medium was MS medium supplemented with 0.5 mg·L<sup>-1</sup> TDZ and 0.2 mg·L<sup>-1</sup> NAA. 1.5 mg·L<sup>-1</sup> 6-BA and 0.2 mg·L<sup>-1</sup> NAA was the optimum hormone combination for differentiation of adventitious buds. And 0.1 mg·L<sup>-1</sup> NAA could efficiently promote rooting. The survival rate of transplants reached about 90%. In this study, using leaves of O. fimbriata as explants, high efficient tissue culture and regeneration system of O. fimbriata were established, and the period from leave to transplant plantlet was about 3 months. The presently developed protocol could be used for large scale clonal propagation and germplasm conservation of O. fimbriata. The efficient tissue culture system of O. fimbriata would provide technical support for its utilization.
This study aimed to investigate the tissue culture and rapid propagation techniques of Orostachys fimbriata, a medicinal and ornamental herbaceous perennial herb belonging to the family Crassulaceae. The leaves of O. fimbriata were used as explants to investigate the effects of plant growth regulators such as thidiazuron (TDZ), 6-benzylamino purine (6-BA) and 1-naphthaleneacetic acid (NAA), on the induction of callus, differentiation of adventitious buds and rooting of shoots. Our results showed that optimum callus induction medium was MS medium supplemented with 0.5 mg·L<sup>-1</sup> TDZ and 0.2 mg·L<sup>-1</sup> NAA. 1.5 mg·L<sup>-1</sup> 6-BA and 0.2 mg·L<sup>-1</sup> NAA was the optimum hormone combination for differentiation of adventitious buds. And 0.1 mg·L<sup>-1</sup> NAA could efficiently promote rooting. The survival rate of transplants reached about 90%. In this study, using leaves of O. fimbriata as explants, high efficient tissue culture and regeneration system of O. fimbriata were established, and the period from leave to transplant plantlet was about 3 months. The presently developed protocol could be used for large scale clonal propagation and germplasm conservation of O. fimbriata. The efficient tissue culture system of O. fimbriata would provide technical support for its utilization.
