摘要
<div style="text-align:justify;"> Polymerase Chain Reaction (PCR) is an accurate, simple and fast analytical method. This technique is widely used in the identification of meat adulteration and meat-based processed food products. Three Mitochondrial DNA (mt-DNA) primers NADH Dehydrogenase sub unit 5 (<em>ND5</em>), <em>D-Loop</em>, and Cytochrome b (<em>Cyt-b</em>) were tested for their specificity in detecting of pig (<em>Sus scrofa</em>) DNA fragments. DNA genome from 6 meat samples (pork, beef, goat, lamb, and chicken) was amplified by PCR technique using three pairs of primers (<em>ND5, D-Loop</em><em>, </em>and <em>Cyt-b</em>) and sequenced. The results of amplification using the three primers produced specific DNA bands with the lengths of 232 bp, 951 bp, and 404 bp, respectively. Comparison results with<em> ND5, D-Loop,</em> and <em>Cyt-b</em> gene sequences resulted in similarity values of 100%, 97%, and 99%, respectively. These showed that the mt-DNA primers of <em>ND5, D-Loop</em>, and<em> Cyt-b </em>genes can be recommended as specific primers in detecting pig (<em>Sus scrofa</em>) DNA fragments. </div>
<div style="text-align:justify;"> Polymerase Chain Reaction (PCR) is an accurate, simple and fast analytical method. This technique is widely used in the identification of meat adulteration and meat-based processed food products. Three Mitochondrial DNA (mt-DNA) primers NADH Dehydrogenase sub unit 5 (<em>ND5</em>), <em>D-Loop</em>, and Cytochrome b (<em>Cyt-b</em>) were tested for their specificity in detecting of pig (<em>Sus scrofa</em>) DNA fragments. DNA genome from 6 meat samples (pork, beef, goat, lamb, and chicken) was amplified by PCR technique using three pairs of primers (<em>ND5, D-Loop</em><em>, </em>and <em>Cyt-b</em>) and sequenced. The results of amplification using the three primers produced specific DNA bands with the lengths of 232 bp, 951 bp, and 404 bp, respectively. Comparison results with<em> ND5, D-Loop,</em> and <em>Cyt-b</em> gene sequences resulted in similarity values of 100%, 97%, and 99%, respectively. These showed that the mt-DNA primers of <em>ND5, D-Loop</em>, and<em> Cyt-b </em>genes can be recommended as specific primers in detecting pig (<em>Sus scrofa</em>) DNA fragments. </div>
作者
Joni Kusnadi
Noval Audi Ashari
Estri Laras Arumingtyas
Joni Kusnadi;Noval Audi Ashari;Estri Laras Arumingtyas(Department of Agricultural Product Technology, Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia;Central Laboratory of Life Sciences, Brawijaya University, Malang, Indonesia;Biology Department, Faculty Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia)
