摘要
In the present investigation antibacterial activity of latexes from ten Indian plant species Spondias dulcis (Amra), Diospyros melanoxylon (Tendu), Terminalia bellirica (Wahera), Ficus glomerata (Gular), Phyllanthus emblica (Awla), Thevetia nerifolia (Kaner), Carica papaya (Papita), Calotropis procera (Ak), Ficus benghalensis (Bargad), Atrocarpus heterophyllus (Kathal) collected from Go-rakhpur, North India were determined in various in vitro systems. MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values were determined in growth inhibitory bioassays by using different increasing concentrations of various latex extracts. Latex samples were diluted by using serial micro dilution method up to 10-10 method with Luria broth culture medium. These values were obtained significantly 2 - 3 times lower than that of broad spectrum antibiotic drugs. Besides this, inhibition zone diameters were measured in agar disc diffusion assay. A known volume i.e. 0.1 - 20 μg/μl of each latex were coated on separate sterile filter paper discs (Whatman No. 1) measuring 6 mm in size. Latex fractions registered significantly higher growth inhibition than that of broad spectrum antimicrobial drugs. Present study indicates the potential use of shows that both latex and its components and latex as are valuable source of medicinal products/active principles that can be used for treatment of life threatening infectious diseases. Because of higher inhibitory and cidal potential obtained in latexes than the synthetic drugs these that could lead to become efficient phytomedicines mainly to have and develop as complete drug formulations against to control infectious microbes.
In the present investigation antibacterial activity of latexes from ten Indian plant species Spondias dulcis (Amra), Diospyros melanoxylon (Tendu), Terminalia bellirica (Wahera), Ficus glomerata (Gular), Phyllanthus emblica (Awla), Thevetia nerifolia (Kaner), Carica papaya (Papita), Calotropis procera (Ak), Ficus benghalensis (Bargad), Atrocarpus heterophyllus (Kathal) collected from Go-rakhpur, North India were determined in various in vitro systems. MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values were determined in growth inhibitory bioassays by using different increasing concentrations of various latex extracts. Latex samples were diluted by using serial micro dilution method up to 10-10 method with Luria broth culture medium. These values were obtained significantly 2 - 3 times lower than that of broad spectrum antibiotic drugs. Besides this, inhibition zone diameters were measured in agar disc diffusion assay. A known volume i.e. 0.1 - 20 μg/μl of each latex were coated on separate sterile filter paper discs (Whatman No. 1) measuring 6 mm in size. Latex fractions registered significantly higher growth inhibition than that of broad spectrum antimicrobial drugs. Present study indicates the potential use of shows that both latex and its components and latex as are valuable source of medicinal products/active principles that can be used for treatment of life threatening infectious diseases. Because of higher inhibitory and cidal potential obtained in latexes than the synthetic drugs these that could lead to become efficient phytomedicines mainly to have and develop as complete drug formulations against to control infectious microbes.
