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Variation of Calcium Oxalate (CaOx) Crystals in Porang Corms (Amorphophallus muelleri Blume) at Different Harvest Time

Variation of Calcium Oxalate (CaOx) Crystals in Porang Corms (Amorphophallus muelleri Blume) at Different Harvest Time
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摘要 Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals;therefore it needs to be observed microscopically. This research used porang corms from the second growing period which were planted to produce the vegetative phase of third growing period. These corms were obtained based on the harvest time had determined. The harvest time was determined, i.e. 1) at two weeks before the plants shed (R<sub>0</sub> - 1);2) when the plants shed (R<sub>0</sub>) and 3) at two weeks after the plants shed (R<sub>0</sub> + 1). Slides for microscopic observation were obtained from slices on the edge and center of porang corms. Organ slices were cleared using modified clearing method. Parameters observed were the shape and the size of CaOx crystal. The variations of styloid, prism, druse and raphide crystals found in porang corms at the three harvest time were 1, 2, 3 and 37 variations respectively. The variation of CaOx crystals tended to be same in porang corms at three harvest times. The variation of these crystals tended to be static. It is also known that raphide crystal has the greatest variation amount. On the contrary, styloid crystal has the fewest variation amounts. This abundance of a number of raphide crystal variations is possibly due to its role as a defense mechanism in porang corms. Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals;therefore it needs to be observed microscopically. This research used porang corms from the second growing period which were planted to produce the vegetative phase of third growing period. These corms were obtained based on the harvest time had determined. The harvest time was determined, i.e. 1) at two weeks before the plants shed (R<sub>0</sub> - 1);2) when the plants shed (R<sub>0</sub>) and 3) at two weeks after the plants shed (R<sub>0</sub> + 1). Slides for microscopic observation were obtained from slices on the edge and center of porang corms. Organ slices were cleared using modified clearing method. Parameters observed were the shape and the size of CaOx crystal. The variations of styloid, prism, druse and raphide crystals found in porang corms at the three harvest time were 1, 2, 3 and 37 variations respectively. The variation of CaOx crystals tended to be same in porang corms at three harvest times. The variation of these crystals tended to be static. It is also known that raphide crystal has the greatest variation amount. On the contrary, styloid crystal has the fewest variation amounts. This abundance of a number of raphide crystal variations is possibly due to its role as a defense mechanism in porang corms.
作者 Nurul Chairiyah Nunung Harijati Retno Mastuti Nurul Chairiyah;Nunung Harijati;Retno Mastuti(Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, Indonesia)
机构地区 Department of Biology
出处 《American Journal of Plant Sciences》 2016年第2期306-315,共10页 美国植物学期刊(英文)
关键词 Harvest Time VARIATION SHAPE CaOx Crystal Porang Corms Harvest Time Variation Shape CaOx Crystal Porang Corms
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