摘要
A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isolate UPMRS4 extracts against P. oryzae and to identify bioactive antifungal compounds produced by UPMRS4. Various solvents were used for extraction of antifungal compounds and well diffusion method was used to determine the antifungal activity of the extracts. The ethyl acetate extract demonstrated the highest activity against mycelial growth of P. oryzae, with an effective inhibitory concentration (EIC) of 1.562 μg/ml significantly higher compared to that of chloroform, diethyl ether, methanol, acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,compounds with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl);Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl);ergotamine;amicomacin;fungichromin;rapamycin and N-Acetyl-D, L-phenylalanine. These compounds had good general antifungal activity and might have potential future agricultural applications.
A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isolate UPMRS4 extracts against P. oryzae and to identify bioactive antifungal compounds produced by UPMRS4. Various solvents were used for extraction of antifungal compounds and well diffusion method was used to determine the antifungal activity of the extracts. The ethyl acetate extract demonstrated the highest activity against mycelial growth of P. oryzae, with an effective inhibitory concentration (EIC) of 1.562 μg/ml significantly higher compared to that of chloroform, diethyl ether, methanol, acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,compounds with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl);Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl);ergotamine;amicomacin;fungichromin;rapamycin and N-Acetyl-D, L-phenylalanine. These compounds had good general antifungal activity and might have potential future agricultural applications.
作者
Hayman Kakakhan Awla
Jugah Kadir
Radziah Othman
Tavga Sulaiman Rashid
Mui-Yun Wong
Hayman Kakakhan Awla;Jugah Kadir;Radziah Othman;Tavga Sulaiman Rashid;Mui-Yun Wong(Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia;Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia;Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Malaysia)
