摘要
<p style="text-align:justify;"> <i><span style="font-family:Verdana;">Background</span></i><span style="font-family:;" "=""> <i><span style="font-family:Verdana;">and</span></i> <i><span style="font-family:Verdana;">aim</span></i><span style="font-family:Verdana;">: Hepatorenal toxicity is a very common ailment with </span><span style="font-family:Verdana;">resultant</span><span style="font-family:Verdana;"> deleterious burden on the overall body systems and high mortality rate. Although myriads of drug agents are in circulation, its medical management is still inadequate as no effective treatment which inhibits disease progression and </span><span style="font-family:Verdana;">complications,</span> <span style="font-family:Verdana;">has</span><span style="font-family:Verdana;"> been </span><span style="font-family:Verdana;">synthesized</span><span style="font-family:Verdana;"> yet. Therefore, this study focused on the potentials of </span><i><span style="font-family:Verdana;">Kigelia</span></i> <i><span style="font-family:Verdana;">africana</span></i><span style="font-family:Verdana;"> ethanolic leaf extract (KAELE) in preventing hepatorenal toxicity using </span><span><span style="font-family:Verdana;">CCl</span><sub><span style="font-family:Verdana;">4</span></sub></span><span style="font-family:Verdana;"> model of toxicity in rats. </span><i><span style="font-family:Verdana;">Method:</span></i><span style="font-family:Verdana;"> KAELE was subjected to phytochemical screening. Following two-week acclimatization, </span><span style="font-family:Verdana;">thirty-six</span><span style="font-family:Verdana;"> (N = 36) adult male Wistar rats were grouped into six consisting of six animals each (n = 6). Group I was given distilled water as control while groups II to VI received silymarin (100 mg/kg), CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (1 ml/kg), KAELE (100 mg/kg, 200 mg/kg </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> 400 mg/kg) respectively. All groups pre-treated with silymarin and </span></span><i><span style="font-family:Verdana;">Kigelia</span></i><span style="font-family:;" "=""> <i><span style="font-family:Verdana;">africana</span></i><span style="font-family:Verdana;"> ethanol leaf extract lasted for a period of fourteen (14) days using a gastric tube. CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> was administered intraperitoneally to groups II, III, IV, V </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> VI 48 hours after the last pretreatment on day 14. </span><span style="font-family:Verdana;">Post treatment</span><span style="font-family:Verdana;">, animals were sacrificed and the blood obtained and sera </span><span style="font-family:Verdana;">used</span><span style="font-family:Verdana;"> for biochemical analysis while the tissues for histological evaluations. </span><i><span style="font-family:Verdana;">Results</span></i><span style="font-family:Verdana;">: </span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">The phytochemical tests revealed the presence of flavonoids, tannins, steroids, terpenoids, saponins, glycosides, alkaloids, and phenols. There was a significant decrease (P < 0.05) in the level of all serum liver enzymes (AST, ALT </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> ALP) in the </span><span style="font-family:Verdana;">extract-treated</span><span style="font-family:Verdana;"> groups. KAELE showed a dose-dependent </span><span style="font-family:Verdana;">hepato-protective</span><span style="font-family:Verdana;"> property as it significantly mitigated the effects of carbon tetrachloride on the liver function markers studied (total bilirubin, conjugated bilirubin, albumin </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> total protein). KAELE showed </span><span style="font-family:Verdana;">the decrease</span><span style="font-family:Verdana;"> necrotic hepatic plates around the portal areas and damaged blood vessels with less fatty </span><span style="font-family:Verdana;">acids</span><span style="font-family:Verdana;"> infiltrations in this study. </span><i><span style="font-family:Verdana;">Conclusion:</span></i><span style="font-family:Verdana;"> KAELE possesses hepatorenal protective potentials.</span></span> </p>
<p style="text-align:justify;"> <i><span style="font-family:Verdana;">Background</span></i><span style="font-family:;" "=""> <i><span style="font-family:Verdana;">and</span></i> <i><span style="font-family:Verdana;">aim</span></i><span style="font-family:Verdana;">: Hepatorenal toxicity is a very common ailment with </span><span style="font-family:Verdana;">resultant</span><span style="font-family:Verdana;"> deleterious burden on the overall body systems and high mortality rate. Although myriads of drug agents are in circulation, its medical management is still inadequate as no effective treatment which inhibits disease progression and </span><span style="font-family:Verdana;">complications,</span> <span style="font-family:Verdana;">has</span><span style="font-family:Verdana;"> been </span><span style="font-family:Verdana;">synthesized</span><span style="font-family:Verdana;"> yet. Therefore, this study focused on the potentials of </span><i><span style="font-family:Verdana;">Kigelia</span></i> <i><span style="font-family:Verdana;">africana</span></i><span style="font-family:Verdana;"> ethanolic leaf extract (KAELE) in preventing hepatorenal toxicity using </span><span><span style="font-family:Verdana;">CCl</span><sub><span style="font-family:Verdana;">4</span></sub></span><span style="font-family:Verdana;"> model of toxicity in rats. </span><i><span style="font-family:Verdana;">Method:</span></i><span style="font-family:Verdana;"> KAELE was subjected to phytochemical screening. Following two-week acclimatization, </span><span style="font-family:Verdana;">thirty-six</span><span style="font-family:Verdana;"> (N = 36) adult male Wistar rats were grouped into six consisting of six animals each (n = 6). Group I was given distilled water as control while groups II to VI received silymarin (100 mg/kg), CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (1 ml/kg), KAELE (100 mg/kg, 200 mg/kg </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> 400 mg/kg) respectively. All groups pre-treated with silymarin and </span></span><i><span style="font-family:Verdana;">Kigelia</span></i><span style="font-family:;" "=""> <i><span style="font-family:Verdana;">africana</span></i><span style="font-family:Verdana;"> ethanol leaf extract lasted for a period of fourteen (14) days using a gastric tube. CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> was administered intraperitoneally to groups II, III, IV, V </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> VI 48 hours after the last pretreatment on day 14. </span><span style="font-family:Verdana;">Post treatment</span><span style="font-family:Verdana;">, animals were sacrificed and the blood obtained and sera </span><span style="font-family:Verdana;">used</span><span style="font-family:Verdana;"> for biochemical analysis while the tissues for histological evaluations. </span><i><span style="font-family:Verdana;">Results</span></i><span style="font-family:Verdana;">: </span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">The phytochemical tests revealed the presence of flavonoids, tannins, steroids, terpenoids, saponins, glycosides, alkaloids, and phenols. There was a significant decrease (P < 0.05) in the level of all serum liver enzymes (AST, ALT </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> ALP) in the </span><span style="font-family:Verdana;">extract-treated</span><span style="font-family:Verdana;"> groups. KAELE showed a dose-dependent </span><span style="font-family:Verdana;">hepato-protective</span><span style="font-family:Verdana;"> property as it significantly mitigated the effects of carbon tetrachloride on the liver function markers studied (total bilirubin, conjugated bilirubin, albumin </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> total protein). KAELE showed </span><span style="font-family:Verdana;">the decrease</span><span style="font-family:Verdana;"> necrotic hepatic plates around the portal areas and damaged blood vessels with less fatty </span><span style="font-family:Verdana;">acids</span><span style="font-family:Verdana;"> infiltrations in this study. </span><i><span style="font-family:Verdana;">Conclusion:</span></i><span style="font-family:Verdana;"> KAELE possesses hepatorenal protective potentials.</span></span> </p>
作者
Wandiahyel Yaduma Gaiuson
Nachamada Emmanuel Solomon
Neftiya Yaduma
Ibrahim Malgwi Samaila
Dluya Thagriki
Wandiahyel Yaduma Gaiuson;Nachamada Emmanuel Solomon;Neftiya Yaduma;Ibrahim Malgwi Samaila;Dluya Thagriki(Department of Chemistry, School of Life Science, Adamawa State College of Education, Hong Yola, Nigeria;Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria;Department of Zoology, Faculty of Sciences, Adamawa State University, Mubi, Nigeria;Department of Human Physiology, College of Medical Sciences, University of Maiduguri, Borno, Nigeria;Department of Biochemistry, Faculty of Sciences, Adamawa State University, Mubi, Nigeria)