摘要
Present study aims to evaluate antimicrobial and antioxidant activities of the crude methanolic extract and different fractions of the <i><span style="font-family:Verdana;">Epilobium</span></i> <i><span style="font-family:Verdana;">roseum</span></i> <span style="font-family:Verdana;">(Schreb). The extract and fractions were used against pathogenic bacteria (</span><i><span style="font-family:Verdana;">Bacillus</span></i> <i><span style="font-family:Verdana;">subtilis</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Bacillus</span></i> <i><span style="font-family:Verdana;">atrophaeus,</span></i> <i><span style="font-family:Verdana;">Klebsiella</span></i> <i><span style="font-family:Verdana;">pneumonia</span></i> <span style="font-family:Verdana;">and </span><i><span style="font-family:Verdana;">Pseudomonas</span></i> <i><span style="font-family:Verdana;">aeruginosa</span></i><span style="font-family:Verdana;">) and fungal strains (</span><i><span style="font-family:Verdana;">Aspergillus</span></i> <i><span style="font-family:Verdana;">niger</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Aspergillus</span></i> <i><span style="font-family:Verdana;">flavus</span></i><span style="font-family:Verdana;">). The methanolic extract and their sub fraction n-hexane showed a prominent inhibition zone against all bacterial strains but inactive against fungal strains. </span><span style="font-family:Verdana;">The various extracts of </span><i><span style="font-family:Verdana;">Epilobium</span></i> <i><span style="font-family:Verdana;">roseum</span></i> <span style="font-family:Verdana;">(Schreb) from various parts were tested for their antioxidant activity by</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay</span><span style="font-family:;" "=""><span style="font-family:Verdana;">. The IC</span><b><sub><span style="font-family:Verdana;font-size:12px;">50</span></sub></b><span style="font-family:Verdana;"> of the stem and root of methanolic extract and their sub fraction n-hexane showed best activity ranged between 22.73 ± 6.92, 21.49 ± 6.26 and 14.94 ± 3.54, 13.92 ± 1.04 μg/ml compared to another fraction. The results support that </span><i><span style="font-family:Verdana;">Epilobium</span></i> <i><span style="font-family:Verdana;">roseum</span></i> <span style="font-family:Verdana;">can be used as antimicrobial and antioxidant agents. The results support the present study that </span><i><span style="font-family:Verdana;">Epilobium</span></i> <i><span style="font-family:Verdana;">roseum</span></i> <span style="font-family:Verdana;">(Schreb) has a potential source of natural antibacterial, antifungal and antioxidant potentials.</span></span>
Present study aims to evaluate antimicrobial and antioxidant activities of the crude methanolic extract and different fractions of the <i><span style="font-family:Verdana;">Epilobium</span></i> <i><span style="font-family:Verdana;">roseum</span></i> <span style="font-family:Verdana;">(Schreb). The extract and fractions were used against pathogenic bacteria (</span><i><span style="font-family:Verdana;">Bacillus</span></i> <i><span style="font-family:Verdana;">subtilis</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Bacillus</span></i> <i><span style="font-family:Verdana;">atrophaeus,</span></i> <i><span style="font-family:Verdana;">Klebsiella</span></i> <i><span style="font-family:Verdana;">pneumonia</span></i> <span style="font-family:Verdana;">and </span><i><span style="font-family:Verdana;">Pseudomonas</span></i> <i><span style="font-family:Verdana;">aeruginosa</span></i><span style="font-family:Verdana;">) and fungal strains (</span><i><span style="font-family:Verdana;">Aspergillus</span></i> <i><span style="font-family:Verdana;">niger</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Aspergillus</span></i> <i><span style="font-family:Verdana;">flavus</span></i><span style="font-family:Verdana;">). The methanolic extract and their sub fraction n-hexane showed a prominent inhibition zone against all bacterial strains but inactive against fungal strains. </span><span style="font-family:Verdana;">The various extracts of </span><i><span style="font-family:Verdana;">Epilobium</span></i> <i><span style="font-family:Verdana;">roseum</span></i> <span style="font-family:Verdana;">(Schreb) from various parts were tested for their antioxidant activity by</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay</span><span style="font-family:;" "=""><span style="font-family:Verdana;">. The IC</span><b><sub><span style="font-family:Verdana;font-size:12px;">50</span></sub></b><span style="font-family:Verdana;"> of the stem and root of methanolic extract and their sub fraction n-hexane showed best activity ranged between 22.73 ± 6.92, 21.49 ± 6.26 and 14.94 ± 3.54, 13.92 ± 1.04 μg/ml compared to another fraction. The results support that </span><i><span style="font-family:Verdana;">Epilobium</span></i> <i><span style="font-family:Verdana;">roseum</span></i> <span style="font-family:Verdana;">can be used as antimicrobial and antioxidant agents. The results support the present study that </span><i><span style="font-family:Verdana;">Epilobium</span></i> <i><span style="font-family:Verdana;">roseum</span></i> <span style="font-family:Verdana;">(Schreb) has a potential source of natural antibacterial, antifungal and antioxidant potentials.</span></span>
作者
Muhammad Ikram
Niaz Ali
Gul Jan
Farzana Gul Jan
Muhammad Romman
Muhammad Ishaq
Yasir Islam
Naeem Khan
Muhammad Ikram;Niaz Ali;Gul Jan;Farzana Gul Jan;Muhammad Romman;Muhammad Ishaq;Yasir Islam;Naeem Khan(Department of Botany, Hazara University, Mansehra, Pakistan;Department of Botany, Abdul Wali Khan University Mardan, Pakistan;Department of Botany, University of Chitral, Chitral, Pakistan;Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan)
