摘要
Artificial hybridization experiment with the <i>Pinus</i> <i>sylvestris</i> and <i>Pinus</i> <i>mugo</i> putative hybrid individuals and their parents revealed a selective nature of their crossability conditioned by the identical chloroplast DNA (cpDNA) haplotypes of the crossed individuals. Efficiency of hybridization between hybrid individual H1 of <i>P</i>. <i>sylvestris</i> haplotype and <i>P</i>. <i>sylvestris</i> paternal tree was relatively high, as evidenced by 78.03% of filled seeds. On the contrary, the crossing of H1 individual with <i>P</i>. <i>mugo</i> was unsuccessful. Likewise, the crossability of the H2 individual of <i>P</i>. <i>mugo</i> haplotype with the paternal tree <i>P</i>. <i>mugo</i> resulted in 31.15% of filled seeds. A relatively high crossability was characteristic also for the H1 and H2 hybrid trees of different cpDNA haplotypes. A conclusion has been drawn postulating weakened reproduction barrier between hybrid swarm individuals and the paternal species <i>P</i>. <i>sylvestris</i> and <i>P</i>. <i>mugo</i>. Differentiation of the putatively hybrid individuals from the parental species is accompanied by the variation in cpDNA inheritance.
Artificial hybridization experiment with the <i>Pinus</i> <i>sylvestris</i> and <i>Pinus</i> <i>mugo</i> putative hybrid individuals and their parents revealed a selective nature of their crossability conditioned by the identical chloroplast DNA (cpDNA) haplotypes of the crossed individuals. Efficiency of hybridization between hybrid individual H1 of <i>P</i>. <i>sylvestris</i> haplotype and <i>P</i>. <i>sylvestris</i> paternal tree was relatively high, as evidenced by 78.03% of filled seeds. On the contrary, the crossing of H1 individual with <i>P</i>. <i>mugo</i> was unsuccessful. Likewise, the crossability of the H2 individual of <i>P</i>. <i>mugo</i> haplotype with the paternal tree <i>P</i>. <i>mugo</i> resulted in 31.15% of filled seeds. A relatively high crossability was characteristic also for the H1 and H2 hybrid trees of different cpDNA haplotypes. A conclusion has been drawn postulating weakened reproduction barrier between hybrid swarm individuals and the paternal species <i>P</i>. <i>sylvestris</i> and <i>P</i>. <i>mugo</i>. Differentiation of the putatively hybrid individuals from the parental species is accompanied by the variation in cpDNA inheritance.
作者
Miroslav Klobucnik
Martin Galgoci
Peter Bolecek
Dusan Gömöry
Andrej Kormutak
Miroslav Klobucnik;Martin Galgoci;Peter Bolecek;Dusan Gömöry;Andrej Kormutak(Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Nitra, Slovak Republic;Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Nitra, Slovak Re-public;Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic;Faculty of Natural Sciences, Constantine Philosopher University, Nitra, Slovak Republic;Faculty of Forestry, Technical University in Zvolen, Zvolen, Slovak Republic)
