摘要
Triple Negative Breast Cancer (TNBC) is a malignant form of cancer with very high mortality and morbidity. Epithelial to Mesenchymal Transition (EMT) is the most common pathophysiological change observed in cancer cells of epithelial origin that promotes metastasis, drug resistance and cancer stem cell formation. Since the information regarding differential gene expression in TNBC cells and cell signaling events leading to EMT is limited, this investigation was done by comparing transcriptomic data generated by RNA isolation and sequencing of a EMT model TNBC cell line in comparison to regular TNBC cells. RNA sequencing and Ingenuity Pathway Software Analysis (IPA) of the transcriptomic data revealed several upregulated and downregulated gene expressions along with novel core canonical pathways including Sirtuin signaling, Oxidative Phosphorylation and Mitochondrial dysfunction events involved in EMT changes of the TNBC cells.
Triple Negative Breast Cancer (TNBC) is a malignant form of cancer with very high mortality and morbidity. Epithelial to Mesenchymal Transition (EMT) is the most common pathophysiological change observed in cancer cells of epithelial origin that promotes metastasis, drug resistance and cancer stem cell formation. Since the information regarding differential gene expression in TNBC cells and cell signaling events leading to EMT is limited, this investigation was done by comparing transcriptomic data generated by RNA isolation and sequencing of a EMT model TNBC cell line in comparison to regular TNBC cells. RNA sequencing and Ingenuity Pathway Software Analysis (IPA) of the transcriptomic data revealed several upregulated and downregulated gene expressions along with novel core canonical pathways including Sirtuin signaling, Oxidative Phosphorylation and Mitochondrial dysfunction events involved in EMT changes of the TNBC cells.
作者
Elizabeth Cagle
Brent Lake
Anasua Banerjee
Jazmine Cuffee
Narendra Banerjee
Darla Gilmartin
Makaiyah Liverman
Shennel Brown
Erik Armstrong
Santanu Bhattacharya
Somiranjan Ghosh
Tanmoy Mandal
Hirendra Banerjee
Elizabeth Cagle;Brent Lake;Anasua Banerjee;Jazmine Cuffee;Narendra Banerjee;Darla Gilmartin;Makaiyah Liverman;Shennel Brown;Erik Armstrong;Santanu Bhattacharya;Somiranjan Ghosh;Tanmoy Mandal;Hirendra Banerjee(Department of Natural, Health and Human Sciences, Elizabeth City State University Campus of The University of North Carolina, Elizabeth, NC, USA;Department of Biochemistry and Molecular Biology, Mayo College of Medicine and Science, Jacksonville, FL, USA;Department of Physiology and Biomedical Engineering, Mayo College of Medicine and Science, Jacksonville, FL, USA;Departments of Pediatrics and Child Health, College of Medicine, Howard University, Washington, DC, USA)
