摘要
In this study two plasma sources were used for an in vivo treatment of human stratum corneum. The sample preparation was realised with the Cyanoacrylat stripping method, whereby a few layers of corneocytes embedded in the lipid matrix were removed from the skin of healthy volunteers. For the plasma treatment, dielectric barrier discharges with pulse durations in the microsecond as well as in the nanosecond range were applied. A comparison of these sources with respect to their biologically active components including dissipated power, gas and electron temperature, irradiance in the ultraviolet range, ozone and nitric oxide concentration is presented. Furthermore, species generated during plasma treatment on the sample surface like hydrogen peroxide, nitride or nitrate were measured using reflectometry. In addition, safety aspects for both sources were evaluated. Resulting plasma induced changes in the sample composition were investigated through X-ray photoelectron spectroscopy. The main ingredients carbon, oxygen, and nitrogen in addition to minor concentrations of sulphur were considered. A significant influence of the pulse duration on plasma characteristics was shown. A more effective formation of reactive species as well as more intense UV emission for ns-plasma was observed. Based on the determined parameters, both plasma sources are suitable for therapeutic purpose. Furthermore, significant plasma induced changes in the stratum corneum composition were reported, including an increase in nitrogen and oxygen content.
In this study two plasma sources were used for an in vivo treatment of human stratum corneum. The sample preparation was realised with the Cyanoacrylat stripping method, whereby a few layers of corneocytes embedded in the lipid matrix were removed from the skin of healthy volunteers. For the plasma treatment, dielectric barrier discharges with pulse durations in the microsecond as well as in the nanosecond range were applied. A comparison of these sources with respect to their biologically active components including dissipated power, gas and electron temperature, irradiance in the ultraviolet range, ozone and nitric oxide concentration is presented. Furthermore, species generated during plasma treatment on the sample surface like hydrogen peroxide, nitride or nitrate were measured using reflectometry. In addition, safety aspects for both sources were evaluated. Resulting plasma induced changes in the sample composition were investigated through X-ray photoelectron spectroscopy. The main ingredients carbon, oxygen, and nitrogen in addition to minor concentrations of sulphur were considered. A significant influence of the pulse duration on plasma characteristics was shown. A more effective formation of reactive species as well as more intense UV emission for ns-plasma was observed. Based on the determined parameters, both plasma sources are suitable for therapeutic purpose. Furthermore, significant plasma induced changes in the stratum corneum composition were reported, including an increase in nitrogen and oxygen content.
作者
Joanna Hirschberg
Leander Loewenthal
Alexander Krupp
Steffen Emmert
Wolfgang Viöl
Joanna Hirschberg;Leander Loewenthal;Alexander Krupp;Steffen Emmert;Wolfgang Viöl(Department of Sciences and Technology, HAWK University of Applied Sciences and Arts, Gottingen, Germany;Fraunhofer Institute for Surface Engineering and Thin Films IST, Application Center for Plasma and Photonic APP, Gottingen, Germany;Clinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany)
