摘要
Helicobacter pylori is the microbial agent most responsible for gastro-duodenal ulcer and chronic gastritis, which can develop into carcinoma of the stomach. This study was performed in Wad Medani Teaching Hospital, Sudan to detect Helicobacter pylori in stomach samples, and evaluate the performance of the tests used, which were histological stains and PCR. Gastric biopsies were obtained from 105 referred patients during endoscopy, and fixed specimens examined by haematoxylin-eosin and Warthin-Starry silver stains, while DNA was extracted for glmM gene amplification. Epigastric pain was the most common symptom at 78% (82/105) and chronic gastritis recorded with 71% (68/105) of endoscopy results. Warthin-Starry silver stain gave 31% (33/105) as positive for Helicobacter pylori followed by glmM gene 27% (28/105) and haematoxylin-eosin 24% (25/105). The study indicated good performance of histological staining and high specificity of glmM gene in detection of Helicobacter pylori from gastric biopsies.
Helicobacter pylori is the microbial agent most responsible for gastro-duodenal ulcer and chronic gastritis, which can develop into carcinoma of the stomach. This study was performed in Wad Medani Teaching Hospital, Sudan to detect Helicobacter pylori in stomach samples, and evaluate the performance of the tests used, which were histological stains and PCR. Gastric biopsies were obtained from 105 referred patients during endoscopy, and fixed specimens examined by haematoxylin-eosin and Warthin-Starry silver stains, while DNA was extracted for glmM gene amplification. Epigastric pain was the most common symptom at 78% (82/105) and chronic gastritis recorded with 71% (68/105) of endoscopy results. Warthin-Starry silver stain gave 31% (33/105) as positive for Helicobacter pylori followed by glmM gene 27% (28/105) and haematoxylin-eosin 24% (25/105). The study indicated good performance of histological staining and high specificity of glmM gene in detection of Helicobacter pylori from gastric biopsies.
作者
Karima Ali Hamid
Albadawi Abdelbagi Talha
Abdalraheem Ali Babiker
Mohammed Ibrahim Malik
Adam Dawoud Abakar
Omer Mustafa
Elsidig A Saeed
Mohmed Bushra Ahmed
Elhadi Abdalla Ahmed
Karima Ali Hamid;Albadawi Abdelbagi Talha;Abdalraheem Ali Babiker;Mohammed Ibrahim Malik;Adam Dawoud Abakar;Omer Mustafa;Elsidig A Saeed;Mohmed Bushra Ahmed;Elhadi Abdalla Ahmed(Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Al Jouf, Kingdom of Saudi Arabia;Department of Medicine, Faculty of Medicine, University of Gezira, Wad Medani, Sudan;Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan)
