摘要
<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span style="font-family:Verdana;"> Tuberculosis is a health problem in Sudan and may become a greater challenge in the future due to the weakness in infection prevention measures, increase in cases of drug-resistant and the difficulty of diagnosis.</span><b><span style="font-family:Verdana;"> Objective: </span></b><span style="font-family:Verdana;">The aim of this study was to detect </span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis</span></i><span style="font-family:Verdana;"> (MTB) from sputum of clinically suspected patients using the three available techniques.</span><b><span style="font-family:Verdana;"> Methods:</span></b><span style="font-family:Verdana;"> Three</span><b> </b><span style="font-family:Verdana;">hundred participants referred to Wad Madani Tuberculosis Center during 2017-2019 were included. Early morning fresh sputum samples were subjected to </span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis</span></i><span style="font-family:Verdana;"> examination by Ziehl-Neelsen</span><b> </b><span style="font-family:Verdana;">(ZN) stain without concentration, ZN stain with centrifugation and geneXpert assay. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">Of the 300 suspected cases;</span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis </span></i><span style="font-family:Verdana;">detected in 17% (51/300) by ZN stain</span><b> </b><span style="font-family:Verdana;">without concentration, 20% (59/300) by ZN stain with centrifugation and 34% (103/300) by geneXpert. The two techniques of ZN stains possessed 100% specificity and relative differences in sensitivity when compared to geneXpert assay. The significant association observed between ZN stains and geneXpert results indicated validity of ZN techniques for detection. </span><b><span style="font-family:Verdana;">Conclusions:</span></b><i> </i><span style="font-family:Verdana;">The study confirmed that geneXpert is better for identification of </span><i><span style="font-family:Verdana;">Mycobacterium</span></i> <i><span style="font-family:Verdana;">tuberculosis </span></i><span style="font-family:Verdana;">when compared to ZN techniques which are also important for diagnosis in poor places and where the geneXpert assay is not available.
<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span style="font-family:Verdana;"> Tuberculosis is a health problem in Sudan and may become a greater challenge in the future due to the weakness in infection prevention measures, increase in cases of drug-resistant and the difficulty of diagnosis.</span><b><span style="font-family:Verdana;"> Objective: </span></b><span style="font-family:Verdana;">The aim of this study was to detect </span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis</span></i><span style="font-family:Verdana;"> (MTB) from sputum of clinically suspected patients using the three available techniques.</span><b><span style="font-family:Verdana;"> Methods:</span></b><span style="font-family:Verdana;"> Three</span><b> </b><span style="font-family:Verdana;">hundred participants referred to Wad Madani Tuberculosis Center during 2017-2019 were included. Early morning fresh sputum samples were subjected to </span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis</span></i><span style="font-family:Verdana;"> examination by Ziehl-Neelsen</span><b> </b><span style="font-family:Verdana;">(ZN) stain without concentration, ZN stain with centrifugation and geneXpert assay. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">Of the 300 suspected cases;</span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis </span></i><span style="font-family:Verdana;">detected in 17% (51/300) by ZN stain</span><b> </b><span style="font-family:Verdana;">without concentration, 20% (59/300) by ZN stain with centrifugation and 34% (103/300) by geneXpert. The two techniques of ZN stains possessed 100% specificity and relative differences in sensitivity when compared to geneXpert assay. The significant association observed between ZN stains and geneXpert results indicated validity of ZN techniques for detection. </span><b><span style="font-family:Verdana;">Conclusions:</span></b><i> </i><span style="font-family:Verdana;">The study confirmed that geneXpert is better for identification of </span><i><span style="font-family:Verdana;">Mycobacterium</span></i> <i><span style="font-family:Verdana;">tuberculosis </span></i><span style="font-family:Verdana;">when compared to ZN techniques which are also important for diagnosis in poor places and where the geneXpert assay is not available.
作者
Zeinab Hamid Elfaham
Elamin M. Ibrahim
Elhadi A. Ahmed
Abdelrhman E. Mohammed
Bakri Y. M. Nour
Zeinab Hamid Elfaham;Elamin M. Ibrahim;Elhadi A. Ahmed;Abdelrhman E. Mohammed;Bakri Y. M. Nour(Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan;Department of Microbiology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan;Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan)
