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Determination of Binding Parameters of Miramistin to Serum Albumin by the Bioluminescent Method

Determination of Binding Parameters of Miramistin to Serum Albumin by the Bioluminescent Method
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摘要 Bioassay based on the luminescent bacteria was used as a new method for the determination of the kinetic constants of ligand binding to the complexion agent. Miramistin as the cationic surface-active antiseptic and serum blood albumin was taken as a model of interacting agent’s. It was concluded that this approach might have a wide range for applications, namely, in all cases where the ligand is an inhibitor of bacterial luminescence and its complex dose not effect the intensity of this process. Bioassay based on the luminescent bacteria was used as a new method for the determination of the kinetic constants of ligand binding to the complexion agent. Miramistin as the cationic surface-active antiseptic and serum blood albumin was taken as a model of interacting agent’s. It was concluded that this approach might have a wide range for applications, namely, in all cases where the ligand is an inhibitor of bacterial luminescence and its complex dose not effect the intensity of this process.
出处 《Advances in Biological Chemistry》 2014年第5期331-338,共8页 生物化学进展(英文)
关键词 Bioluminescent INHIBITION Test Miramistin ALBUMIN ASSOCIATION CONSTANTS DETERMINATION Bioluminescent Inhibition Test Miramistin Albumin Association Constants Determination
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