摘要
This work studied the thermostability and the biostability of chlorophyll species obtained from an extract of spinach leaves embedded in a silica xerogel matrix. The analysis was done by monitoring the photosystem II (PSII) using fluorescence spectroscopy. Samples were prepared using the sol gel method with a molar ratio of ethanol/H<sub>2</sub>O/TEOS of 4:11.6:1. Then, silica xerogel matrix was loaded with an extract of spinach leaves, obtained in dark conditions. The measurement of the fluorescence spectra was done at selected temperatures to the corresponding non-physiological regimen. Results indicate that the PSII band position remains unchanged when heat treatment temperature increases up 200°C. For temperatures above 100?C, the fluorescence intensity diminishes linearly when the temperature increases. The photosystem II embedded in silica xerogel matrix is decomposed at temperatures above 200°C.
This work studied the thermostability and the biostability of chlorophyll species obtained from an extract of spinach leaves embedded in a silica xerogel matrix. The analysis was done by monitoring the photosystem II (PSII) using fluorescence spectroscopy. Samples were prepared using the sol gel method with a molar ratio of ethanol/H<sub>2</sub>O/TEOS of 4:11.6:1. Then, silica xerogel matrix was loaded with an extract of spinach leaves, obtained in dark conditions. The measurement of the fluorescence spectra was done at selected temperatures to the corresponding non-physiological regimen. Results indicate that the PSII band position remains unchanged when heat treatment temperature increases up 200°C. For temperatures above 100?C, the fluorescence intensity diminishes linearly when the temperature increases. The photosystem II embedded in silica xerogel matrix is decomposed at temperatures above 200°C.
作者
José Martínez-Mendoza
Erika Espericueta-González
Diana Espericueta González
Gerardo Ortega-Zarzosa
Azdrubal Lobo Guerrero
José Martínez-Mendoza;Erika Espericueta-González;Diana Espericueta González;Gerardo Ortega-Zarzosa;Azdrubal Lobo Guerrero(Departamento de Físico-Matemáticas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México;Facultad de Ciencias, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México;Facultad de Ingeniería, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México;Doctorado Institucional de Ciencia e Ingeniería de Materiales, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México;Instituto de Física, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México)