摘要
The development of the spectrofluorimetric method can be considered a promising alternative that is relatively less expensive and sufficiently reliable. In the current literature, no method for the analysis of nevirapine by spectro-fluorimetric has been reported. The proposed method is based on the transformation of naturally non-fluorescent nevirapine into a fluorescent derivative after chemical synthesis. Maximum excitation and emission wavelengths are 290 nm and 357 nm respectively. The analytical performance of the method demonstrates linearity in the concentration range 1.5 × 10<sup>-2</sup> and 13.5 × 10<sup>-2</sup> μg/mL with a correlation coefficient (r) greater than 0.999. The detection (LOD) and quantification (LOQ) limits found are 1.97 × 10<sup>-3</sup> μg/mL and 5.48 × 10<sup>-3</sup> μg/mL respectively. Recovery is achieved with 99.9% and 100.3% trueness, intra-day precision with a coefficient of variation of repeatability (CVr) of 0.99% and inter-day precision with a coefficient of variation of precision (CVR) of 1.7%. The method has been successfully applied in the analysis of 10 batches of nevirapine tablets and suspensions.
The development of the spectrofluorimetric method can be considered a promising alternative that is relatively less expensive and sufficiently reliable. In the current literature, no method for the analysis of nevirapine by spectro-fluorimetric has been reported. The proposed method is based on the transformation of naturally non-fluorescent nevirapine into a fluorescent derivative after chemical synthesis. Maximum excitation and emission wavelengths are 290 nm and 357 nm respectively. The analytical performance of the method demonstrates linearity in the concentration range 1.5 × 10<sup>-2</sup> and 13.5 × 10<sup>-2</sup> μg/mL with a correlation coefficient (r) greater than 0.999. The detection (LOD) and quantification (LOQ) limits found are 1.97 × 10<sup>-3</sup> μg/mL and 5.48 × 10<sup>-3</sup> μg/mL respectively. Recovery is achieved with 99.9% and 100.3% trueness, intra-day precision with a coefficient of variation of repeatability (CVr) of 0.99% and inter-day precision with a coefficient of variation of precision (CVR) of 1.7%. The method has been successfully applied in the analysis of 10 batches of nevirapine tablets and suspensions.
作者
Lucien Murhula Namegabe
Aladin Ombeni Mahano
Serigne Omar Sarr
Lucien Murhula Namegabe;Aladin Ombeni Mahano;Serigne Omar Sarr(Official University of Bukavu, Faculty of Pharmaceutical Sciences and Public Health, Bukavu, Bukavu, Democratic Republic of the Congo;Cheikh Anta Diop University, Analytical Chemistry, and Food Sciences Laboratory, Dakar, Senegal;National Medecines Control Laboratory, Dakar, Senegal)
