摘要
Possibility of wood biomass for preparing organic soil was examined to construct reproducible and stable organic standard soil. Seven organic soils were constructed from base soils and additive materials based on the recommended values of the soil fertility index (SOFIX) (total carbon ≥ 25,000 mg/kg, total nitrogen ≥ 1500 mg/kg, total phosphorus ≥ 1100, and total potassium of 2500 to 10,000 mg/kg). Base soils were prepared from two types of wood biomass (big- and small-sized wood chips) at 50%, 60%, and 70% (v/v) and other organic materials such as peat moss, black soil, and mountain soil. Additive materials (soybean meal, oil cake, cow manure, and bone meal) were amended into all organic soils at the same amount. Incubation experiment showed that bacterial biomass in all organic soil was greater than 6 × 108 cells/g-soil after addition of 30% of water content for 1 week. In addition, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) analysis resulted in a stable bacterial diversity of the organic soil prepared from the small size wood chip at 70%. Chemical properties of all organic soils were within the recommended values of SOFIX. The plant cultivation experiment showed that fresh Brassica rapa var. peruviridis weights in the organic soils with 50%, 60%, and 70% of small-sized wood chip were 5%, 16%, and 27% higher than that of the chemical fertilizer-amended soil. The organic soil with 70% of small wood chip was the best in the seven organic soils in this study.
Possibility of wood biomass for preparing organic soil was examined to construct reproducible and stable organic standard soil. Seven organic soils were constructed from base soils and additive materials based on the recommended values of the soil fertility index (SOFIX) (total carbon ≥ 25,000 mg/kg, total nitrogen ≥ 1500 mg/kg, total phosphorus ≥ 1100, and total potassium of 2500 to 10,000 mg/kg). Base soils were prepared from two types of wood biomass (big- and small-sized wood chips) at 50%, 60%, and 70% (v/v) and other organic materials such as peat moss, black soil, and mountain soil. Additive materials (soybean meal, oil cake, cow manure, and bone meal) were amended into all organic soils at the same amount. Incubation experiment showed that bacterial biomass in all organic soil was greater than 6 × 108 cells/g-soil after addition of 30% of water content for 1 week. In addition, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) analysis resulted in a stable bacterial diversity of the organic soil prepared from the small size wood chip at 70%. Chemical properties of all organic soils were within the recommended values of SOFIX. The plant cultivation experiment showed that fresh Brassica rapa var. peruviridis weights in the organic soils with 50%, 60%, and 70% of small-sized wood chip were 5%, 16%, and 27% higher than that of the chemical fertilizer-amended soil. The organic soil with 70% of small wood chip was the best in the seven organic soils in this study.
