摘要
Natural extracellular matrices (ECMs) perform the tasks necessary for tissue formation, maintenance, regulation and function, providing a powerful means of controlling the biological performance of regenerative materials. In addition, biomedical materials have claimed attention because of the increased interest in tissue engineering materials for wound care and regenerative medicine. Moreover, the nanostructure and morphological similarities with collagen make BC attractive for cell immobilization, cell support and Natural Extracellular Matrix (ECM) Scaffolds. In this work, we present the extracellular matrix (ECM) using the bacterial cellulose (Nanoskin<sup>®</sup>) which regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Bacterial cellulose fermentation process is modified before the bacteria are inoculated for mimicking ECM to cells support and built new local material for wound healing. Chemical groups influences and thermal behavior in bacterial cellulose were analyzed using transmission infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), respectively. Besides, In vivo analysis was evaluated with clinical study at Sharjah Kuwait Hospital.
Natural extracellular matrices (ECMs) perform the tasks necessary for tissue formation, maintenance, regulation and function, providing a powerful means of controlling the biological performance of regenerative materials. In addition, biomedical materials have claimed attention because of the increased interest in tissue engineering materials for wound care and regenerative medicine. Moreover, the nanostructure and morphological similarities with collagen make BC attractive for cell immobilization, cell support and Natural Extracellular Matrix (ECM) Scaffolds. In this work, we present the extracellular matrix (ECM) using the bacterial cellulose (Nanoskin<sup>®</sup>) which regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Bacterial cellulose fermentation process is modified before the bacteria are inoculated for mimicking ECM to cells support and built new local material for wound healing. Chemical groups influences and thermal behavior in bacterial cellulose were analyzed using transmission infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), respectively. Besides, In vivo analysis was evaluated with clinical study at Sharjah Kuwait Hospital.
作者
Saqer Al Mualla
Raed Farahat
Pierre Basmaji
Gabriel Molina de Olyveira
Ligia Maria Manzine Costa
José Domingos da Costa Oliveira
Gino Bruno Francozo
Saqer Al Mualla;Raed Farahat;Pierre Basmaji;Gabriel Molina de Olyveira;Ligia Maria Manzine Costa;José Domingos da Costa Oliveira;Gino Bruno Francozo(Al Qassimi Hospital/Kuwait Hospital, Sharjah, United Arab Emirates;Innovatec’s-Biotechnology Research and Development, São Carlos, Brazil;Department of Physical Chemistry, Universidade Estadual Paulista (UNESP), Araraquara, Brazil;Materials Engineering Department, São Carlos School of Engineering, University of São Paulo, São Carlos, Brazil)
