摘要
At the moment<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">,</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> we see a great interest for application of Anti Sense Oligonucleotides</span><span style="font-family:Verdana;"> (ASOs) </span><span style="font-family:Verdana;">in order to regulate the expression of genes related to certain diseases. These nucleotides obtained a number of fascinating properties by means of chemical manipulation of natural DNA and RNA under conservation of Watson-Crick base-pairing. About 35 years ago for our research in this field</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">,</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> we selected synthetically (short) </span><i><span style="font-family:Verdana;">phosphate-methylated</span></i><span style="font-family:Verdana;"> DNA and RNA. It was concluded that there is an exclusive selection in hybridization affinity with natural DNA and RNA. These (bio)chemical and physical-chemical properties are extensively published. ASOs have found their </span><span style="font-family:Verdana;">way in public health as is clearly shown in the treatment of (progressive)</span><span style="font-family:Verdana;"> neurological diseases. We focus specifically on the past, present and future of the </span><span style="font-family:Verdana;">phosphate-methylated oligonucleotides, illustrated with different research</span><span style="font-family:Verdana;"> stu</span><span style="font-family:Verdana;">dies in chemistry and biophysics. A new field of application of modified</span><span style="font-family:Verdana;"> DNAs is based on interactive improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing phosphate-methylated DNA as probe.</span></span></span></span>
At the moment<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">,</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> we see a great interest for application of Anti Sense Oligonucleotides</span><span style="font-family:Verdana;"> (ASOs) </span><span style="font-family:Verdana;">in order to regulate the expression of genes related to certain diseases. These nucleotides obtained a number of fascinating properties by means of chemical manipulation of natural DNA and RNA under conservation of Watson-Crick base-pairing. About 35 years ago for our research in this field</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">,</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> we selected synthetically (short) </span><i><span style="font-family:Verdana;">phosphate-methylated</span></i><span style="font-family:Verdana;"> DNA and RNA. It was concluded that there is an exclusive selection in hybridization affinity with natural DNA and RNA. These (bio)chemical and physical-chemical properties are extensively published. ASOs have found their </span><span style="font-family:Verdana;">way in public health as is clearly shown in the treatment of (progressive)</span><span style="font-family:Verdana;"> neurological diseases. We focus specifically on the past, present and future of the </span><span style="font-family:Verdana;">phosphate-methylated oligonucleotides, illustrated with different research</span><span style="font-family:Verdana;"> stu</span><span style="font-family:Verdana;">dies in chemistry and biophysics. A new field of application of modified</span><span style="font-family:Verdana;"> DNAs is based on interactive improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing phosphate-methylated DNA as probe.</span></span></span></span>
作者
Henk M. Buck
Henk M. Buck(Kasteel Twikkelerf 94, Tilburg, The Netherlands)
