摘要
Lipid-rich extracts were obtained from brown algae Saccharina cichorioides, Costaria costata, Chorda filum, Eularia fistulosa, Dictyopteris divaricata, Dictyosiphon chordaria, Silvetia babingtonii, and Fucus evanescens that were collected in Peter the Great Gulf of the Sea of Japan. The ability of algalextracts and glyceroglycolipids (GLs) monogalactosyldiacylglycerols (MGDG), digalactosyldiacylglycerols (DGDG), and sulfoquinovosyldiacylglycerols (SQDG) and carotenoid fucoxanthin to suppression of reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells, studied. The results showed that algae extracts could suppress ROS. However, extracts of D. divaricata, D. chordaria, C. filum, S. babingtonii, and F. evanescens had a higher degree of suppression of ROS. Extracts of S. cichorioides and D. divaricata showed the dependence of their activity from the month of collecting these algae. The GLs and fucoxanthin were isolated from extracts of using column chromatography with silica gel and their ROS-inhibitory activity was investigated too. The fatty acids (FAs) composition of lipids was determined byGC and GC/MS. It has been found that MGDG and DGDG stronger than SGDG inhibited the ROS and the degree of their activity depended on the species of algae, the month of collection, the amount of PUFA, the ratio of n - 3 and n - 6 PUFA in GLs. Fucoxanthin has shown a high degree of suppression of ROS. This preliminary study has shown the prospect of a deeper study of the suppression of ROS with the help of lipids from algae the Sea of Japan.
Lipid-rich extracts were obtained from brown algae Saccharina cichorioides, Costaria costata, Chorda filum, Eularia fistulosa, Dictyopteris divaricata, Dictyosiphon chordaria, Silvetia babingtonii, and Fucus evanescens that were collected in Peter the Great Gulf of the Sea of Japan. The ability of algalextracts and glyceroglycolipids (GLs) monogalactosyldiacylglycerols (MGDG), digalactosyldiacylglycerols (DGDG), and sulfoquinovosyldiacylglycerols (SQDG) and carotenoid fucoxanthin to suppression of reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells, studied. The results showed that algae extracts could suppress ROS. However, extracts of D. divaricata, D. chordaria, C. filum, S. babingtonii, and F. evanescens had a higher degree of suppression of ROS. Extracts of S. cichorioides and D. divaricata showed the dependence of their activity from the month of collecting these algae. The GLs and fucoxanthin were isolated from extracts of using column chromatography with silica gel and their ROS-inhibitory activity was investigated too. The fatty acids (FAs) composition of lipids was determined byGC and GC/MS. It has been found that MGDG and DGDG stronger than SGDG inhibited the ROS and the degree of their activity depended on the species of algae, the month of collection, the amount of PUFA, the ratio of n - 3 and n - 6 PUFA in GLs. Fucoxanthin has shown a high degree of suppression of ROS. This preliminary study has shown the prospect of a deeper study of the suppression of ROS with the help of lipids from algae the Sea of Japan.
