姜黄素对哮喘小鼠体内NF-κB、IκB及p-IκB含量的影响

Effect of curcumin on NF-κB 、IκB、p-IκB in asthmatic mice

目的 了解姜黄素对哮喘小鼠核因子(NF)-κB、IκB、p-IκB的含量的影响.方法 对30只Balb/c小鼠随机分组,分为正常对照组、哮喘组及姜黄素干预哮喘小鼠.应用卵清蛋白溶液建立哮喘小鼠模型;对各组小鼠进行肺功能的检测;检测各组肺组织中的NF-κB、IκB及p-IκB的含量.结果 哮喘组胞浆内NF-κB较对照组减少(P＜0.01),而姜黄素干预组胞浆内NF-κB含量较哮喘组明显增加,具有统计学意义(P＜0.05).哮喘组胞核内NF-κB较对照组明显增加(P＜0.01),而姜黄素干预组胞核内NF-κB较哮喘组明显减少,差异具有统计学意义(P＜0.05).哮喘组胞浆内的p-IκB含量显著高于对照组(P＜0.01),而姜黄素干预组胞浆内的p-IκB含量显著低于哮喘组,差异具有统计学意义(P＜0.01).哮喘组胞浆内的IκB含量显著低于对照组(P＜0.01),而姜黄素干预组IκB含量较哮喘组显著增高,差异具有统计学意义(P＜0.01).结论 姜黄素通过减轻IκB的磷酸化抑制NF-κB转录入核内,从而减轻哮喘小鼠的气道高反应性.

Objective To investigate the effect of the asthmatic mice's contents of NF-κB、IκB、p-IκB treated with curcumin.Methods Thirty Balb/c mice were divided into three groups randomly,the normal mice,the asthmatic mice and the curcumin mice.The bronchial hyperresponsiveness of the three groups were exanined by lung function.The NF-κB 、IκB 、p-IκB content of three teams were tested.Results The content of cytoplasm NF-κB in asthmatic mice was lower than the control (P ＜ 0.01).However,the content of cytoplasm NF-κB in curcumin team was more than the asthmatic mice (P ＜ 0.05).In the other hand,The content of nuclear NF-κB in asthmatic mice was more than the control(P ＜0.01).However,the content of nuclear NF-κB in curcumin mice was lower than the asthmatic mice (P ＜ 0.05).The content of cytoplasm p-hκB in asthmatic mice was more than the control and the content of IκB was lower than the control(P ＜ 0.01).Howerer,the content of IκB in curcumin team have much more than the asthmatic mice and the content of p-IκB have much lower than the control(P ＜ 0.01).Condusion Curcumin alleviates the airway hyperresponsiveness of the asthmatic mice through the suppression of NF-κB transcribe to the nuclear via alleviating the phosphorylation of I-κB.