小尺度连续切片三维重建技术研究进展

Research progress of small-scale serial sections-based 3D reconstruction technologies

三维重建技术广泛应用于辅助医学诊断、手术模拟和解剖教学等领域.计算机断层扫描(CT)、磁共振成像(MRI)等传统影像学数据的分辨率并不能满足生命科学研究的需求.连续切片技术可提供绝大部分组织的精准高分辨率信息,对显微结构的研究和探索是一种非常有价值的方法.随着显微镜及图像处理技术的发展,小尺度连续切片的三维重建技术取得了重大发展,对样本形态和组织功能的分析成为可能,并且可对组织结构进行更完整的评估,在细胞和组织生物学领域引起了深刻的变革.就常见小尺度连续切片三维重建技术进行了比较和综述.

3D reconstruction technologies have been widely used in medical diagnostic assist, surgical simulation, anatomy teaching, etc. The resolution of traditional imaging data such as computed tomography (CT) and magnetic resonance imaging (MRI) cannot meet the needs of life science research. Continuous sectioning technology can provide accurate and high-resolution information for the vast majority of tissues, which make it become a valuable method in the research and exploration of microstructures studies. With the development of microscopy and image processing techniques, significant advances have been made in the small-scale serial sections-based 3D reconstruction technologies. This enables the analysis of sample morphology and tissue function, as well as a more complete assessment of the structure of the tissue. Therefore, this technology has led to profound changes in the fields of cell and tissue biology. In this paper, the common small-scale serial sections-based 3D reconstruction technologies were compared and reviewed.