SLC5A8基因转录沉默与乳腺癌

Transcriptional silencing of SLC5A8 gene in breast cancer

目的 探讨乳腺癌发生过程中的SLC5A8基因转录沉默现象.方法 回顾性分析2014年6月-2015年6月河北大学附属医院基本外科收治的32例乳腺癌患者的病例资料,分析术中采集的乳腺癌组织及癌旁组织标本,采用实时荧光定量聚合酶链式反应法检测两组标本中SLC5A8基因的转录水平.应用配对t检验和x2检验的方法分析结果.结果 癌组织SLC5A8基因mRNA转录水平显著低于癌旁组织(P＜0.05).乳腺癌组织中有20例(62.5％) SLC5A8基因无转录产物.癌旁组织标本中仅有4例(12.5％) SLC5A8无基因转录产物.癌组织的SLC5A8的失活率显著高于癌旁组织(P＜0.05).结论 SLC5A8基因转录沉默是乳腺癌发生过程中的重要事件.

Objective To explore the phenomenon of gene silence of SLC5A8 in breast cancer.Methods Thirty-two patients suffered with breast cancer,hospitalized from June 2014 to June 2015 in affiliated hospital of Hebei university,were collected with the cancer tissue and normal specimen.Real-time quantitative PCR method was used to detect SLC5A8 gene transcription.Paired t-test and-test were used to anaylsed the results.Results Twenty cases of breast cancer tissues (62.5％) have no SLC5A8 gene transcription product,normal breast tissue adjacent to carcinoma specimens that had no product gene transcription was only five (12.5％).SLC5A8 inactivation rate of cancer tissue was significantly higher than normal tissue adjacent to carcinoma (P ＜ 0.05).The SLC5A8 mRNA transcription level of cancer tissue was significantly lower than the normal (P ＜ 0.05).Conclusion SLC5A8 gene transcription silence is the important events in the process of breast cancer occurrence and development.