NINJ2基因多态性与陕西地区脑梗塞患者的相关性的研究

Association study of NINJ2 gene polymorphism and cerebral infarction patients in Shaanxi area

目的 :探讨神经损伤诱导蛋白2(NINJ2)基因多态性与陕西地区缺血性脑卒中的关系。方法 :选取2014年1月~2016年2月在我院治疗的缺血性脑卒中患者172例作为观察组,同时选取在我院进行体检的110例健康人作为对照组,采用聚合酶联反应-限制性片段长度多态性技术对NINJ2 rs10849373、rs4980840、rs2535393和rs11063806位点进行基因型检测,比较观察组和对照组基因型和等位基因频率的差异。结果 :观察组体重指数(BMI)、血清总胆固醇(TC)和低密度脂蛋白(LDL)分别为(25.22±1.89)kg/m^2、(4.81±1.22)mmol/L和(3.01±0.82)mmol/L,吸烟和饮酒的比例分别为40.70%和27.91%,均高于对照组,差异比较有统计学意义;rs10849373、rs4980840、rs2535393和rs11063806位点GG、GA和AA基因型在观察组和对照组的频率分布符合Hardy-Weinberg平衡定律;观察组rs10849373位点AA、GA基因型以及等位基因A的频率分别为11.63%、40.12%和31.69%,明显高于对照组,rs10849373位点的基因型及基因频率在观察组和对照组间差异有统计学意义;rs4980840、rs2535393和rs11063806位点在观察组和对照组间的分布差异无统计学意义。结论 :NINJ2基因中rs10849373位点与陕西地区缺血性脑卒中有关,需进一步研究。

Objective To investigate the relationship between the Nerve injury induced protein 2 gene(NINJ2)polymorphism and ischemic stroke in Shaanxi area. Methods From January 2014 to February 2016, 172 cases of ischemic stroke patients in our hospital were selected as observation group, at the same time, 110 healthy persons were selected as the control group, used polymerase chain reaction-restriction fragment length polymorphism to dected NINJ2 gene rs10849373, rs4980840, rs2535393 and rs11063806 locus genotype, compared the differences of genotype and allele frequencies between the observation group and the control group. Results Observation group body mass index(BMI), serum total cholesterol(TC)and low density lipoprotein(LD L)were(25.22±1.89)kg/m2, (4.81±1.22)mmol/L and(3.0±0.82)mmol/L, the proportion of smoking and drinking were 40.70%and 27.91%, were higher than the control group, the difference had statistical significance;rs10849373, rs4980840, rs2535393 and rs11063806 locus GG, AA and AA genotype frequency distribution accords with the Hardy-Weinberg equilibrium in the observation group and control group;Observation group rs10849373 locus AA and GA genotype and allele A frequency were 11.63%, 40.12%and 31.69%, significantly higher than the control group, rs10849373 locus genotype and gene fre-quency differences between the observation group and the control group had statistical significance;rs4980840, rs2535393 and rs11063806 locus distribution difference between the observation group and the control group was not statistically significant . Conclusion NINJ2 gene rs10849373 locus is associated with ischemic stroke in Shaanxi area, which needs further study.