液基细胞学检查DNA定量分析在宫颈癌早期筛查中的应用研究及意义

Application and significance of liquid-based cytology and DNA quantitative analysis in early screening of cervical

目的 :观察宫颈细胞DNA与液基细胞检查在宫颈癌早期筛查中的应用效果。方法 :取宫颈癌早期筛查患者12630例,入院后患者均行宫颈细胞DNA倍体定量检测和液基细胞学联合测定完成早期筛查。对1146例宫颈癌细胞DNA倍体定量测定或液基细胞学筛查阳性患者进行阴道镜检查并进行组织病理学检测。结果 :1728例DNA定量分析阳性,占13.68%;1133例液基细胞学检查阳性,占8.97%。DNA定量分析中10902例未见,1063例DNA细胞少量异常;液基细胞学检查中11497例正常或炎症;575例为不典型鳞状上皮细胞,365例低度鳞状上皮内病变;1146例液基细胞学检查DNA定量分析异常行阴道镜检查活检,5阳性率为50.61%,诊断敏感性为89.14%(517/580),特异性为75.80%(429/566);1070例液基细胞学检查阳性,以宫颈活检为标准,液基细胞学检查敏感性为73.28%(425/780),特异性为76.50%(433/566)。结论 :液基细胞检查与宫颈细胞DNA定量分析均为早期宫颈癌常用的筛查方法,且两者联合筛查能提高诊断敏感性、特异性,值得推广应用。

Objective To observe the effect and value of liquid-based cytology combined with DNA quantitative analysis in the early screening of cervical cancer. Methods 12, 630 patients with cervical cancer were screened for early screening. All patients were examined by DNA ploidy and liquid - based cytology. The positive rate of DNA ploidy and liquid-based cytol-ogy in 1146 cases of cervical cancer were examined by colposcopy and histopathological examination. The results of liquid-based cytology combined with DNA quantitative analysis in the early screening of cervical cancer . Results DNA quantitative analysis was positive in 1728 cases, accounting for 13.68%.1133 cases were positive by liquid-based cytology, accounting for 8.97%. DNA quantitative analysis of 10902 cases, 1063 cases of DNA cells a small amount of abnormal; liquid-based cytology in 11497 cases of normal or inflammation; 575 cases of atypical squamous epithelial cells, 365 cases of low-grade squamous intraepithelial lesions; 1146 cases The positive rate was 50.61%, the diagnostic sensitivity was 89.14% (517/580), the specifi-city was 75.80% (429/566); 1070 cases of liquid-based cytology The sensitivity was 73.28% (425/780), and the specificity was 76.50% (433/566). Conclusion The sensitivity and specificity of liquid-based cytology examination are 73.28% (425/780) and 76.50% (433/566) respectively. Conclusion: Both liquid-based cytology test and DNA quantitative analysis of cervical cells are common screening methods for early cervical cancer, and combined screening can improve the sensitivity and specificity of di-agnosis. It is worthy of popularization and application.