高浓度CO_2对采后杏鲍菇乙醛和乙醇含量及其相关酶活性的影响

Effects of elevated CO_2 on aldehyde and ethanol contents and the activities of related enzymes in postharvest Pleurotus eryngii

为了探讨高浓度CO_2对采后杏鲍菇生理变化的调控机理,首先研究了不同薄膜包装气调处理对杏鲍菇采后品质的影响。结果表明,低O_2(2%~4%)结合高浓度CO_2(30%~40%)处理可减缓采后杏鲍菇的衰老。进一步采用人工气调处理分析了2%O_2+30%CO_2对杏鲍菇乙醛、乙醇和丙酮酸含量,以及丙酮酸脱羧酶、乙醇脱氢酶和乳酸脱氢酶活性的影响。结果显示,与对照相比,2%O_2+30%CO_2处理提高了杏鲍菇中乙醛、乙醇和丙酮酸含量,并增强了丙酮酸脱羧酶、乙醇脱氢酶和乳酸脱氢酶的活性。可见,2%O_2+30%CO_2的高浓度CO_2处理是一种延长杏鲍菇贮藏期限的有效采后处理方法,高浓度CO_2的这种作用可能与其诱发的厌氧代谢有关。

In order to elucidate the adjustment mechanism of elevated CO2 on the physiological changes of postharvest Pleurotus eryngii, the effects of different film package on postharvest quality of Pleurotus eryngii were firstly investigated.The results showed that the combination of O2 (2%-4%) and CO2 (30%-40%) could reduce the senescence of postharvest Pleurotus eryngii.Furtherly, the effects of controlled atmosphere treatment (2% O2 and 30% CO2) on the content of aldehyde, ethanol and pyruvic acid, and the activities of pyruvate decarboxylase, alcohol dehydrogenase and lactate dehydrogenase of Pleurotus eryngii were analyzed.The results indicated that the treatment of 2% O2 and 30% CO2 raised the content of ethanol, aldehyde and pyruvic acid, and increased the activities of pyruvate decarboxylase, alcohol dehydrogenase and lactate dehydrogenase of Pleurotus eryngii.These results suggest that the treatment of elevated CO2 (2% O2 and 30% CO2) is a promising postharvest strategy for Pleurotus eryngii to extent storage time, and the effect of elevated CO2 is interrelated with anaerobic metabolism caused by itself.