老年鼠运动预干预产生脑缺血再灌注损伤后神经保护作用的血源性机制研究

The mechanism of neuroprotective effects induced by pre-exercise

目的探讨运动预干预产生脑缺血再灌注损伤后神经保护作用的血源性机制。方法 SD雄性老年大鼠36只随机分为运动预干预(S-EX)组和非运动(S-NEX)组,每组18只;S-EX组给予6个月跑台运动训练,干预后每组取6只老年大鼠进行蛋白质谱分析,其余大鼠建立脑缺血模型进行脑损伤程度评价。结果 S-EX组大鼠体重低于S-NEX组,神经缺损优于S-NEX组,对长期运动预干预后的大鼠血清进行蛋白质谱分析,筛选得到63个运动相关的差异蛋白,其中结合珠蛋白与其他蛋白相互作用关系密切,S-EX组老年大鼠肝脏中结合珠蛋白基因的mRNA表达水平高于S-NEX组(P<0.01)。结论运动预干预产生的脑缺血损伤后神经保护作用可能与血清结合珠蛋白水平升高有关。

Objective To investigate the mechanism of neuroprotective effects induced by pre-exercise.Methods Totally 36 senile SD rats were randomly divided into exercise group(S-EX) and non-exercise group(S-NEX).Exercise group was given six months of treadmill training.After intervention,6 rats from each group were executed for blood collection and protein profiling,and the others were used to generate cerebral ischemia blood brain models for evaluation of the extent of damage.Results Body weight of rats in S-EX group was significantly lower than the S-NEX group,while neurological deficit was significantly better than non-exercise group.The protein profiling screened 63 sport-related differences in proteins,.Haptoglobin protein closely interact with other proteins.S-EX liver gene Haptoglobin mRNA expression was significantly higher than S-NEX group.Conclusion Neuroprotective effect induced by pre-exercise may be related to elevated levels of serum protein Haptoglobin