Hippo通路相关蛋白在卵巢癌组织中的表达及其临床意义

Expression and Clinical Significance of Hippo Signaling Molecules in Ovarian Carcinoma

目的检测卵巢癌组织中Mst1、Lats1、Yap1三种蛋白的表达,分析其与临床病理参数的相关性,探讨Hippo通路在卵巢恶性肿瘤发生中的作用。方法应用免疫组化技术测定正常卵巢组织、卵巢癌组织(包括浆液性囊腺癌、粘液性囊腺癌、卵巢子宫内膜样癌)中Mst1、Lats1及Yap1的表达情况,并分析其与临床病理参数的相关性。结果 (1)Mst1、Lats1在卵巢癌中的阳性表达率均低于正常卵巢组织(χ2=7.027,P=0.007;χ2=16.896,P=0.000);Yap1在卵巢癌中的阳性表达率高于正常卵巢组织(χ2=17.725,P=0.000)。(2)Mst1、Lats1及Yap1的阳性表达率与卵巢癌分化程度及临床分期相关(P<0.05),而与患者肿瘤病理类型、是否淋巴结转移无关(P>0.05)。(3)卵巢癌组织中Mst1、Lats1与Yap1的表达呈负相关(r分别为-0.380、-0.541,P均<0.05),Mst1与Lats1的表达呈正相关(r=0.166,P<0.05)。结论 Hippo信号通路中Mst1、Lats1、Yap1在卵巢癌的发生发展中起重要作用,并有望为卵巢癌的临床治疗提供新的靶点和可靠依据。

Objective To observe the expression of Mstl,Latsl and Yap1 in epithelial ovarian cancer and to analyze their correlation with clinical pathology parameters,and to explore the mechanism of Hippo pathway in ovarian malignant tumor. Methods Immunohistochemistry was used to investigate the expression of Mstl,Latsl and Yap1 in normal ovarian tissue,serous cystadenocarcinoma,mucinous cystadenocarcinoma,and endometrioid tumor. The relationship between these three proteins and pathology parameters was analyzed. Results(1)The positive rates of Mstl and Latsl expression in ovarian carcinoma were significantly lower than those in the normal control group,(Mstl χ2=7.027,P=0.007;Latsl χ2=16.896,P=0.000). The positive rate of Yap1 expression in ovarian cancer was significantly higher than those in the normal control group(χ2=17.725,P=0.000).(2)The positive rates of Mstl,Latsl and Yapl expression had a significant correlation with pathological grading and tumor differentiation(P<0.05). However they had no significant correlation with histological type and lymph node metastasis(P>0.05).(3)The positive rate of Yap 1 expression had a significantly negative correlation with those of Mstl and Latsl expression in ovarian cancer(r=-0.380,-0.541),while Mstl expression had a significantly positive correlation with Latsl(r=0.166). Conclusion The Hippo signaling pathway may play an important role in the development of ovarian carcinoma. And it is expected to provide new targets and reliable basis for early diagnosis,prevention,clinical treatment and prognosis judgement of ovarian cancer.