摘要
Objective: To identify Kadsura interior(Dian Ji Xue Teng, Schisandraceae) by using DNA barcoding.Methods: We analyzed five DNA barcodes(ITS, ITS2, psb A-trn H, mat K and rbc L) using DNA barcoding in terms of distance-based,tree-based and character-based identification to distinguish Kadsura interior and its adulterants.Results: In distance-based and tree-based identification, K. interior could be distinguished easily from the species of Schisandra and K. coccinea.In character-based identification, there are two single nucleotide polymorphisms(SNPs) in ITS and one SNP in psb A-trn H which can be used to distinguish K. interior from K. heteroclita and K. longipedunculata.Conclusion: The results indicate that DNA barcoding can be used to identify K. interior. ITS and psb A-trnH sequence can be the most ideal DNA barcode for discriminating K. interior and its adulterants by the combination analysis of distance-based, tree-based and character-based identification(SNPs).
Objective: To identify Kadsura interior (Dian Ji Xue Teng, Schisandraceae) by using DNA barcoding. Methods: We analyzed five DNA barcodes (ITS, ITS2, psbA-trnH, matK and rbcL) using DNA barcoding in terms of distance-based, tree-based and character-based identification to distinguish Kadsura interior and its adulterants. Results: In distance-based and tree-based identification, K. interior could be distinguished easily from the species of Schisandra and K. coccinea. In character-based identification, there are two single nucleotide polymorphisms (SNPs) in ITS and one SNP in psbA-trnH which can be used to distinguish K. interior from K. heteroclita and K. longipedunculata. Conclusion: The results indicate that DNA barcoding can be used to identify K. interior. ITS and psbA-trnH sequence can be the most ideal DNA barcode for discriminating K. interior and its adulterants by the combination analysis of distance-based, tree-based and character-based identification (SNPs).
基金
the support of the National Natural Science Foundation of China(Project No.81373913)
