甘蔗汁多酚氧化酶的提取研究

The Extraction of Polyphenol Oxidase from Sugarcane Juice

以磷酸盐缓冲液匀浆浸提法提取混合甘蔗汁多酚氧化酶(PPO),探讨缓冲液p H值、表面活性剂添加量和聚乙烯吡咯烷酮(PVPP)添加量对甘蔗汁PPO提取效果的影响。结果表明,以邻苯二酚为底物时,获得甘蔗汁PPO的酶活性最适测定波长是230 nm。磷酸盐缓冲液提取甘蔗汁PPO最适p H值为6.2;在磷酸盐缓冲溶液中PVPP最适添加量为2%,提取甘蔗汁PPO的比活性最高。在缓冲液中加入表面活性剂Tween 80和Triton X-100时,提取甘蔗汁PPO的比活性较低。

Polyphenol oxidase (PPO) was extracted from mix sugarcane juice by phosphate buffer homogenate extraction. The effects of pH value, surfactant adding quantity and polyvinyl polypyrrolidone (PVPP) adding quantity on the PPO extraction were determined. The results showed that the optimal wavelength of enzymatic activity of PPO was 230 nm when catechol was used as substrate. The optimal pH value of PPO was 6.2. The op-timal adding quantity of PVPP was 2%in phosphate buffer solution, and the highest specific activity of PPO was obtained in sugarcane juice. When adding surfactant Tween 80 and Triton X-100 in buffer, the specific ac-tivity of PPO in extracting sugarcane juice was low.