羊肚菌发酵豆渣生产多糖及其功能性的研究

Utilization of Soybean Curd Residue for Polysaccharides Production by Morchella esculenta and Evaluation of the Functional Properties

以豆渣为培养基,利用羊肚菌进行半固体发酵,通过正交试验优化发酵条件,得到最佳的发酵条件为料液比1∶9(g/m L),接种量3.0%,发酵时间9 d,发酵产物中多糖(CMP,Crude Morchella Polysaccharide)含量达到8.86%。同时,对CMP的功能性进行评价,结合胆酸盐能力试验表明,CMP对3种胆酸盐(无水胆酸盐、脱氧胆酸盐、牛磺胆酸盐)均有结合效果,其中对脱氧胆酸钠的结合量最高,为(0.16±0.02)mmol/L,证明CMP有降血脂功能。体外抗氧化试验表明,CMP在浓度为10 mg/m L时对羟自由基的清除能力达98.9%,显示其具有良好的抗氧化性。

The semisolid fermentation by Morchella esculenta using soybean curd residue was studied. Orthogo-nal experimental design was employed to optimize the fermentation conditions. The optimal fermentation condi tions achieved for crude polysaccharide production 8.86%were feed liquid ratio 1:9 (g/mL), inoculum size 3.0%and fermentation time 9 days,respectively. The crude polysaccharide (CMP) was extracted from the fer-mentation substrate,then the function of CMP was evaluated. The results on the ability of bile salt-binding showed that CMP had binding capacities for three kinds of cholates (anhydrous cholate, deoxycholate, tauro-cholate),which demonstrated that CMP had hypolipidemic effect. Meanwhile, CMP exhibited a positive antioxi-dant activity.