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核酸检验与酶联免疫在检测血液病毒中的应用分析 被引量:6

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摘要 近年来通过输血途径感染血液病毒如艾滋病(HIV)、乙型肝炎(HBV)、丙型肝炎(HCV)的患者逐年增多,对患者造成严重伤害和影响。究其根本原因就是血液病毒检测准确性差[1]。目前使用酶联免疫法(ELISA)检测血液病毒较为普遍,但是这一检测方法有明显的局限性,受检测仪器和环境的影响较大。我院近年来主要采用核酸检验法(NAT)对血液病毒进行检测,阳性检出率较高,现报道如下。
作者 赫英飞
出处 《中国现代医药杂志》 2016年第10期73-75,共3页 Modern Medicine Journal of China
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  • 1许文燕,邱茂锋,佐合拉.吐尔地,邢文革,蒋岩.第四代HIV抗原抗体酶联检测试剂缩短HIV检测窗口期的研究[J].中华检验医学杂志,2007,30(3):284-287. 被引量:63
  • 2Schottstedt V, Tuma W, Bringer G, et al. Routine PCR screening for HBV, HCV and HIV-I genome in a large blood donation services-experiences and initial results [J]. Beitr Infusionsther Transfusionsmed, 1997,34 : 21-25.
  • 3Roth WK, Busch MP, Sehuller A, et al. International survey on NAT testing of blood donations: expanding implementation and yield from 1999 to 2009[J]. VoxSang, 2012,102(1): 82-90.
  • 4Gallarda JL, Dragon E. Blood screening by nucleic aci amplification teehnology current issues future chanllenges[J]. Mol Diagn, 2000,5 (1) : 11-22.
  • 5Meng Q, Wong C, Rangachari A, et al. Automated multiplex assay system for sumutaneous detection of hepatitis B virus DNA, hepatitis C virus RNA, and human immunodefieiency virus type 1 RNA[J]. Clin Mierobiol, 2001,39(2) :2937-2945.
  • 6Allain JP. Occult hepatitis B virus :infection: implications in transfusion[J]. Vox Sang, 2004, 86(2) :83-91.
  • 7何亚琴,张建伟,杨爱龙,等.核酸检测技术在常州地区献血筛查中的应用[J].2011:24(7):560—562.
  • 8Mine H,Emra HM. High throughout screening of 16 million serologically negative bloods donors for hepati- tis t3 virus,and human immunodeficiency virus type-1 by nucleic acid amplification testing with specific and sensitive multiplex reagent in Japan[J]. J Virol Meth- ods, 2003,112 (1-2) : 145-151.
  • 9Jarvis L, 13ecker J,Tender A,et al. Evaluation of the Roche cobas 201 system and cobas TaqScreen multi- plex test for blood screening: a European multicenter study[J].Transfusion,2008,48 (9) :1853-1861.
  • 10Margaritis AR, Brown SM, Seed CR, et al. Comparison of two automated nucleic acid testing systems for si- multaneous detection of human immunodeficiency virus and hepatitis C virus RNA and hepatitis B virus DNA [J]. Transfusion,2007,47(lO):1783-1793.

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