摘要
Objective: This paper aims at measurement enhanced effect of oxidized lipoprotien(a) [ox Lp(a)] on permeability of monolayer endothelial cells and relationship with reactive oxygen species(ROS) generation and desmogleins(DSGs) expression.Methods and Results: Transendothelial permeability was assayed by transwell and reactive oxygen species(ROS) was determined by DCFH-DA staining. RT-PCR was carried out to determine DSGl and DSC2 expression in m RNA, respectively.Transendothelial permeability was enhanced by ox LP(a) dose and time dependently. The most marked effect appeared at a concentration of 100 mg/L, Transendothelial permeability reached the maximum value after 2 h of FITC-dextran addition, and then gradually decreased after 4 h. ox Lp(a) induces the generation of cellular reactive oxygen species(ROS), and this effect could be inhibited by superoxide dismutase(SOD).Incubation of HUVECs with ox Lp(a) resulted in a dose and time-dependent down-regulation of DSGl and DSC2 expression at transcriptional level. Conclusion:Permeability of monolayer endothelial cells was enhanced by ox Lp(a) which is related to up-regulating ROS formation and down-regulating desmogleins expression.
Objective: This paper aims at measurement enhanced effect of oxidized lipoprotien(a) [oxLp(a)] on permeability of monolayer endothelial cells and relationship with reactive oxygen species(ROS) generation and desmogleins(DSGs) expression. Methods and Results: Transendothelial permeability was assayed by transwell and reactive oxygen species (ROS) was determined by DCFH-DA staining. RT-PCR was carried out to determine DSGl and DSC2 expression in mRNA, respectively. Transendothelial permeability was enhanced by oxLP (a) dose and time dependently. The most marked effect appeared at a concentration of 100 mg/L, Transendothelial permeability reached the maximum value after 2 h of FITC-dextran addition, and then gradually decreased after 4 h. oxLp (a) induces the generation of cellular reactive oxygen species (ROS), and this effect could be inhibited by superoxide dismutase (SOD). Incubation of HUVECs with oxLp (a) resulted in a dose and time-dependent down-regulation of DSGl and DSC2 expression at transcriptional level. Conclusion: Permeability of monolayer endothelial cells was enhanced by oxLp(a) which is related to up-regulating ROS formation and down-regulating desmogleins expression.
基金
Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province,National and Natural Science Foundation of China
grant number:81070221
Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University) of Ministry of Education(2010)
