乙型肝炎患儿外周血中Th17细胞频率的特征及意义

Characteristics of Th17 cells in peripheral blood in paediatric patients with chronic hepatitis B

目的 研究慢性乙型肝炎患儿外周血Th17细胞频率的特征,并探讨其与部分临床指标的相关性.方法 本研究入组2010年12月至2014年2月于泰安市妇幼保健院收治的45例16岁以下乙型肝炎患儿,取患儿外周血检测乙型肝炎表面标志物(HBV-M)、乙型肝炎病毒核酸载量(HBV-DNA)、丙氨酸氨基转移酶(ALT)及外周血Th17细胞的频率,并对患儿肝组织进行病理分级.另选1 1名健康儿童为对照组.结果 与对照组[(1.61±0.56)％]相比,乙型肝炎患儿外周血Th17细胞频率[(3.03±1.08)％]显著增高(t=-0.52,P＜0.01);乙型肝炎患儿外周血Th17细胞频率与HBV DNA载量[lg(HBV DNA)6.49±3.25]和ALT水平[(40.5±11.3) U/L]无相关性(P＞0.05),但肝脏病理分级高的乙型肝炎患儿(G2、G3)外周血Th17细胞频率[(5.95±2.05)％]显著高于肝脏病理分级低的乙型肝炎患儿(G0、G1)[(1.31±0.19)％],差异有统计学意义(t=-1.51,P＜0.01).结论 乙型肝炎患儿外周血Th17细胞频率与肝脏损伤程度密切相关.

Objective To study the characteristics of Th17 cells frequency and the relationship with clinical characters in paediatric patients with chronic hepatitis B.Methods Forty-five paediatric patients with chronic hepatitis B within 16-year old from December 2010 to February 2014 in Maternal and Child Health-Care Hospital were enrolled in present study,and 11 age-matched healthy children were enrolled as control group.Hepatitis B virus markers(HBV-M),HBV-DNA loads and alanine amino shift enzyme(ALT) levels in serum were detected in the two groups.The frequencies of Thl7 cells in peripheral blood were measured by flow cytometry.Tumor grade was classed in Children with liver tissue.Results Compared with the control group ((1.61 ± 0.56) ％),the Th17 cells frequencies in peripheral blood ((3.03 ± 1.08) ％) were significantly increased in chronic hepatitis B paediatric patients(t=-0.52,P＜0.01).There was no correlations between Th17 cells frequencies with HBV DNA quantity(lg(HBV-DNA)6.49±3.25) and ALT level((40.5±11.3) U/L) (P ＞0.05).The patients with higher HAI scores (G2-G3) had a greater proportion of Th17 cells ((5.95 ±2.05)％) than those with lower HAI scores(G0-G1) ((1.31±0.19)％),and the difference was significant(t =-1.51,P＜0.01).Conclusion Th17 cells frequencies in peripheral blood were associated closely with liver injury in paediatric patients with CHB.