微量酶联免疫吸附试验检测人群弓形体IgG抗体

DETECTION OF SERUM ANTIBODY IgG OF TOXOPLASMA GONDII BY MICRO-ELISA

弓形体病是由弓形体引起的一种细胞内寄生人畜共患的流行性传染病,目前国内仍以间接血凝法(IHA)为最主要的诊断方法.1976年Voller等[1]应用微量酶联免疫吸附法(Micro-ELISA)测定弓形体抗体,取得了较好结果[2].我们将该法加以改良(改良法),检测了北京地区人群血清中弓形体IgG抗体,报告如下.

Serum antibody IgG of Toxoplasma gondii in 192 normal subjects was simultaneouslytested by micro-ELISA and indirect hema gglutination(Ittk).Among them 22 werepositve(11.46%)by mioro-ELISA and 18 positive by IHA(9.37%).The coincident rateof the two methods was 93.75%.The preparation of antigen was specially studied.Asoitiofluid containing large amount of trophozoites was freeze-thawed once and then washedthree times to eradicate the unwanted cells and proteins,followed by ultrasonication tobreak up the trophozoite8.The antigen was then ultraoentrifuged and filtrated throughELISA gave strong specifloity and high sensitivity,and the amount of serum required wasonly 10m1.It is practically better than IHA,and a desirable method in epidemiologioalsurvey of Toxoplasmosis.