摘要
本文应用免疫荧光染色技术及组织培养技术,结合细胞学检查,研究了流行性出血热(EHF)病毒对骨髓细胞的作用.检测了51例患者骨髓细胞的病毒抗原,37例呈现阳性.抗原从第一病日即出现,持续至第十一病日.用VeroE6细胞从10份患者骨髓细胞中分离病,10份全部阳性.细胞学观察见巨核细胞有成熟障碍,并在细胞胞浆扩张的内质网及高尔基氏体腔内观察到到病毒样颗粒.根据实验结果,认为EHF病毒可侵入骨髓细胞中,并可能引起巨核细胞成熟障碍及免疫细胞发育异常.
The antigen of epidemic hemorrhagic fever(EHF) virus was found in bone marrow cells of 37 EHF patients by the aid of IFA.The specificity of the antigen was confirmed by McAb staining.Bone marrow cells of 21 patients with other diseases were also studied as contro1.Virus antigen was seen from the first day till the eleventh day of EHF.EHF virus was successfully isolated from the Suspension of bone marrow cells of 10 patients with EHF and serially passaged.Megakaryocyte mature obstacle and virus like particles in dilated channel of rough endoplasmic reticulum and Golgi apparatus were observed under the light and electron transmission microscopy.It is considered that EHF virus could attack human bone marrow cells and probably cause damage of the cells.
出处
《中华传染病杂志》
CAS
1987年第1期19-22,插页2,共5页
Chinese Journal of Infectious Diseases
