斑点杂交试验直接检测血清HBV DNA在乙型肝炎诊断中的应用

APPLICATION OF DIRECT SPOT HYBRIDIZATION TO SERUM HBV DNA IN HEPATITIS B PATIENTS

本文用斑点杂交试验直接检测149例不同类型的乙型肝炎血清中HBV DNA,其阳性率为65.8%(98/149),各型间无差异.其中116例检测了DNAP,阳性率为62%(72/116),HBV DNA阳性率为72.4%(841/116).在116例中HBsAg、HBeAg和抗-HBc均阳性者54例,其DNAP和HBVDNA阳性率分别为79.6%(43/54)和92.6%(50/54); HBsAg、抗-HBe和抗-I-IBc均阳性者35例,DNAP和HBV DNA阳性率分别为37.1%(13/35)和60%(21/35);HBsAg和抗-HBc阳性者27例,DNAP和HBV DNA阳性率分别为59.2%(16/27)和 48.1%(13/27).同时有6份供血员血清阴性.结果表明,本法高度特异、敏感、简便,可作为判断病毒复制的手段.

One hundred forty-nine patients with different types of hepatitis B were assayed for HBV DNA With direct spot hybridization.HBV DNA positive tale was 65.8%(98/149).There was no signflcant difference among various types.DNAP was tested in 116 of the 149 cases.the Positive rate Was 62%(72/116).and HBV DNA positive rate was 72.4%(s4/116).Of them.DNAP and HBV DNA positive rates were 79.6 %(43/54)and 92.6 %(50/54)respeotively in 54 cases with positive HBsAg,HBeAg and anti-HBc.DNAP and HBV DNA Positive rates were 37.1%(13/35)and 60%(21/35)respectively in 35 cases with posilive HBSAg,anti-HBe and anit-HBc.DNAP and HBV DNA positive rates were 59.2%(16/27) and 4S.1%(13/27)respectively in 27 cases with positive HBsAg and anti-HBc.In addition, these markers Were negative in 6 healthy blood donors. The results indicate that the new method for detecting serum HBV DNA is highly specific,sensitive and simple,and could be used as a more direct technique to re3ognize active viral replication.