乙型肝炎患者肝细胞膜脂蛋白循环免疫复合物的研究

LIVER-SPECIFIC CIRCULATING IMMUNE COMPLEX IN VIRAL HEPATITIS B

本文采用亲和层析法纯化的肝细胞膜特异性脂蛋白(LSP)制备抗血清并提取IgG.用ELISA间接法测定乙型肝炎94例患者血清的LSP特异性复合物(ICLSP)并同时测定乙型肝炎表面系统免疫复合物(ICHBS)及C3补体含量.发现血液中ICLSP,含量以重型肝炎和慢性活动性肝炎组中为高,在此类患者中工ICLSP的阳性检出率高于ICHBS的阳性率.在ICLSP阳性病例中多伴有C3补体的消耗,ICLSP可能是引向重型肝炎肝持续损害原因之一,ICLSP的形成可能是继发于肝组织损伤,LSP渗入血液内的结果.

The anti-LSP-IgG was prepared from tbe rabbit antiserum against LSP which was isolated from human embryo liver and purified with the affinity chromatography technique. By means of the ELISA detecting system and the anti-LSP-IgG as the coated antigen,the cirenlatJng immune complex ICnsP was studied in the 94 patiens with variouS types of viral hepatitis B.The content of ICLSP was increased in the serum of fulminant and ehronic active hepatitis,and the percentage ef positive ICLSP was found higher than those of positive ICHBs. We also found that the complement fraction 3 was consumed in the ICLsP positive oases.It seems that IQLSp plays an important role in elieiting the protracted deterioration in fulminant hepatitis and subaeut liver necrosis.Perhaps the ICLSP may be resulted from the liver tissue damage from which the LSP penetrating into the blood.