甲型与乙型肝炎病毒双重感染时免疫组织化学的检测

IMMUNOHISTOCHEMICAL ASSAY FOR HBsAg AND HBcAg IN HBV AND HAV DOUBLE INFECTION BY MONOCLONAL ANTIBODY AND ABC METHOD ZRU CHuNWU

30例血清抗-HAV-IgM阳性的急性病毒性肝炎患者中检出21例(70%)HAV和HBV双重感染,其中混合感染5例(16.67%),病理诊断均为急性轻型肝炎,血清学检出2例抗HBc-IgM阳性,免疫组织化学检出3例肝组织内HBcAg阳性.重叠感染16例(53.33%),血清学证实HBV感染15例,免疫组织化学检出HBsAg和/或HBcAg阳性者10例,其中一例血清学HBV各项标志物均阴性,而肝HBsAg阳性.免疫组化检测提高了双重感染的检出率.

Dept of PathoZogy,The 302 Hospital,PLAThe immunohistochemical assays for HBsAg and HBcAg in liver sections of 30 cases with serunn anti-HAV IgM positive acute hepatitis were performed.Twenty'one cases(70.0%)of HAV and HBV double infection were detected.Five cases of coinfection were histopathologically diagnosed as acute hepatitis,of which 3 showed serum HBV markers negative but HBcAg positive in liver sections.Sixteen cases of superinfection were hisfopathologically diagnosed as CAH in 7,CPH in 5,ACH in 2 and involution changes in 2 cases.Three of them were HBsAg or HBcAg positive in liver sections in acute phase of HAV infectJon.but serum HBV markers were negative.The results showed that the expression of HBV markers might be inhibited when double infection occurred.The immunohistochemical assay for HBsAg and HBcAg could increase the rate of eliciting the double infection.