乙型肝炎患者血清HBV DNA和e系统相关性的研究

STUDY ON RELATIONSHIP BETWEEN HBV DNA AND e SYSTEM IN HEPATITIS B PATIENTS

本文对172例乙型肝炎患者应用斑点杂交方法测定血清HRVDNI.在HBeAg阳性患者中血清HBVDNA检出率为90.10%;而在HBsAg阳性,但HBeAg和抗HBe均为阴性或抗HBe阳性患者中血清HBVDNI检出率分别为23.52%和28.13%以上结果提示HBeAg和血清HBVDNA密切相关,且反映了病毒的活动性复制,而抗HBc阳性并不意味着病毒复制已停止和不再有传染性.根据我们的资料还可看出HBeAg和血清HBVDNA同时阳性者多见于无症状HBsAg携带者、急性肝炎和慢性迁延性肝炎患者.与此相反,抗-HBe和血清HBVDNA同时阳性者则多见于慢性活动性肝炎和肝硬化患者.由此可见,HBeAg和血清HBVDNA的不一致性与肝脏病变的进展程度有关.

We have assessed the presence of HBV DNA in serum samples from 172 patients with HBsAg-positive hepatitis B by hybridiization test with cloned HBV DNA probe.It was found that the positive rates of serum HBV DNA were 90.10%,28.13% and 23.52% in HBeAg positive,anti-I-tBe positire and e system negative patients respectively.The results indicate that there is a close correlation between serum HBV DNA and e system.The serum HBV DNA is more sensitive than e system in reflecting the replication of HBV.On the other hand,the data showed that the model of both positive HBV DNA and HBeAg was often seen in HBsAg carrier,acute hepatitis and CPH,while the model of both positive HBV DNA and anti-HBe often seen in cirrhosis and GAH.It seems tbat there are some relationships between the aforementioned different models an d various degrees of severity of hepatitis.