慢性乙型肝炎患者自然杀伤细胞和抗体依赖性细胞毒活性降低的机理探讨

AN APPROACH TO THE DECREASE ACTIVITY OF NATURAL KILLER CELL AND ANTIBODY-DEPENDANT CELL-MEDIATED CYTOTOXICITY IN CHRONIC HEPATITIS B

本文观察35例慢性乙型肝炎患者外周血单核细胞中HBV标志物和血清中的细胞毒抑制因子.结果表明,15例低自然杀伤细胞(NK)活性患者中3例HBsAg(+),1例HBeAg(+);12例HBV DNA(+);10例低抗体依赖性细胞毒(ADCC)活性患者中8例HBsAg(+),1例HBeAg(+),8例HEV DNA(+).对血清细胞毒抑制因子初步的理化性质分析,观察到两种不同的抑制成分;两种因子都能抑制正常人的细胞毒活性各玫瑰花环形成.提示慢性乙型肝炎患者的细胞毒活性降低可能与HBV对NK和K细胞的损害及患者血清中的某些抑制因子有关.

The HBV markers of peripheral blood mononuclear cells and serum cytotoxity inhibitory factors were obsenved in 35 patienis with chronic hepatitis B.Natural killer cells(NK)acitivity was decreased in 15 cases with 3 were of HBsAg(+),one HBeAg(+)and 12 HBV DNA(+).Antibody-dependant cell-mediated cytotoxicity(ADCC)activity Was decreased in 10 cases with 3 were of HBsAg(+),one HBeAg (+) and 8 HBV DNA (+).Preliminary study on the physical and chemical char8cterics of serum inhibitory factors showed that there were two different types.One was found similar to RIF,which could nog be dialysed and stable at 56℃ or at-20℃.The other one could be dialysed and neither stable to heat nor to cold.Both factors could inhibit the oytoxicity and E rosette formation.These findings suggest that the NK and K cells were damaged and the presence of certain inhibitory factors in chronic HBV infection.