Yes相关蛋白在非小细胞肺癌组织中的表达及其临床意义

Expression and clinical significance of Yes-associated protein in non-small cell lung cancer

目的：探讨 Yes 相关蛋白（YAP）在非小细胞肺癌组织中的表达与临床病理特征的关系及其在非小细胞肺癌发生发展中的作用。方法采用实时定量反转录多聚酶链反应（RT-PCR）、免疫组织化学及 Western blot 方法，分别检测非小细胞肺癌患者的癌组织、正常肺组织中 YAP 蛋白的表达情况，采用 SPSS 17.0统计软件包进行统计学分析，计数资料比较采用卡方检验，应用非配对 t 检验检测非小细胞肺癌组织和正常肺组织中 YAP mRNA 表达差异，以 P＜0.05为差异有统计学意义。结果非小细胞肺癌组 YAP 阳性率（65%）高于正常肺组织组（5%）（χ2=43.2，P=0.000）。YAP 阳性率与非小细胞肺癌的病理分级（高、中、低分化阳性率分别为35.9%、67.6%和87.2%）密切相关（χ2=24.837，P=0.000）；淋巴结转移者 YAP 的表达率（76.1%）与无淋巴结转移者（58.1%）相比差异也有统计学意义（χ2=4.03，P=0.045）。YAP mRNA 在非小细胞肺癌组织中的相对表达量（0.4013±0.0783）是正常肺组织（0.0499±0.0084）的8.04倍，差异具有统计学意义（t=3.21，P＜0.001）。结论 YAP 可能参与了非小细胞肺癌的发生和发展的过程；可能成为判断非小细胞肺癌发生发展和评价预后的有效参考指标之一，对于非小细胞肺癌的早期预测可能具有重要的临床意义。

Objective To investigate the expressive significance of YAP in non-small cell lung cancer (NSCLC) with clinicopathologic features and its role in the occurrence and development of NSCLC. Methods The expression of YAP protein in NSCLC and normal lung tissue was detected by real-time reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and Western blot. Analysis of data was performed using SPSS 17.0. The chi-square test was applied for different comparison among enumeration data. The difference of YAP mRNA expression in NSCLC tissue and normal lung tissue was detected by unpaired t test. The level of significance was set at P<0.05. Results The positive rate of YAP was higher in NSCLC group (65%) than that in normal lung (5%) tissue (χ2=43.2, P=0.000). In NSCLC, the expression of YAP was related to the pathological grade (high differentiation vs. middle differentiation vs. low differentiation: 35.9% vs. 67.6% vs. 87.2%; χ2=24.837, P=0.000). The expression of YAP in group with lymph node metastasis (76.1%) was also significantly different from that in group without lymph node metastasis (58.1%) (χ2=4.03, P=0.045). The relative expression of YAP mRNA in NSCLC tissue (0.401 3±0.078 3) was 8.04 times of normal lung tissue (0.049 9±0.008 4). The difference was statistically significant (t=3.21, P<0.001). Conclusions The results indicate that YAP may participate in the process of occurrence and progression in NSCLC. Detection of YAP may be an effective parameter in evaluating occurrence, progression and prognosis of NSCLC, and may also have important clinical significance in early prediction of NSCLC.