目的探讨cyc lin D1、p27k ip1在普通型肾细胞癌(renal cell carc inom a,RCC)发生、发展中的作用。方法用半定量RT-PCR方法检测25例普通型RCC和10例肿瘤远端的正常肾组织中cyc lin D1的mRNA含量,用免疫组化方法检测76例普通型RCC中cyc ...目的探讨cyc lin D1、p27k ip1在普通型肾细胞癌(renal cell carc inom a,RCC)发生、发展中的作用。方法用半定量RT-PCR方法检测25例普通型RCC和10例肿瘤远端的正常肾组织中cyc lin D1的mRNA含量,用免疫组化方法检测76例普通型RCC中cyc lin D1和p27k ip1蛋白的表达,并对cyc lin D1、p27k ip1蛋白表达与临床病理参数的关系进行分析。结果普通型RCC中cyc lin D1的mRNA含量0.488±0.399,高于正常对照组0.089±0.066(P<0.01)。cyc lin D1的表达与肿瘤体积大小有关,体积大者cyc lin D1高表达(P<0.05)。普通型RCC中p27k ip1表达低于正常对照组,随着p27k ip1表达的降低,肿瘤的细胞核Fu-hrm an分级、TNM分期增高。结论cyc lin D1高表达和p27k ip1的低表达与普通型RCC的发生有关;p27k ip1的低表达可能促进肿瘤的演进,p27k ip1的表达可作为评价普通型RCC预后的参考指标。展开更多
网络直播带货是近几年新兴的线上购物方式,在数据收集流通、购物支付方式以及购物体验等方面实现对传统购物的升级,同时也成为网络直播变现的另一主要途径。在当前网络平台具有虚拟性、互动性、广泛性的情况下,直播平台的法律责任认定...网络直播带货是近几年新兴的线上购物方式,在数据收集流通、购物支付方式以及购物体验等方面实现对传统购物的升级,同时也成为网络直播变现的另一主要途径。在当前网络平台具有虚拟性、互动性、广泛性的情况下,直播平台的法律责任认定存在直播虚拟化、功能定位不准确、以及法律依据不健全等难题,需要通过提高技术手段、建立监管制度、完善相关法律等方式明确直播带货平台的法律责任,否则将对电商行业带来难以消除的负面影响。Online live streaming sales is a new way of online shopping in recent years. It has upgraded traditional shopping in terms of data collection and circulation, shopping payment methods and shopping experience, and has also become another main way to realize online live streaming. In the current network platform with virtual, interactive, extensive, live platform of legal liability that live virtualization, function positioning is not accurate, and the legal basis is not sound, need to improve technical means, establish regulatory system, improve the relevant legal way clear live with goods platform of legal responsibility, otherwise will bring difficult to eliminate the negative impact of the electricity industry.展开更多
文摘目的探讨cyc lin D1、p27k ip1在普通型肾细胞癌(renal cell carc inom a,RCC)发生、发展中的作用。方法用半定量RT-PCR方法检测25例普通型RCC和10例肿瘤远端的正常肾组织中cyc lin D1的mRNA含量,用免疫组化方法检测76例普通型RCC中cyc lin D1和p27k ip1蛋白的表达,并对cyc lin D1、p27k ip1蛋白表达与临床病理参数的关系进行分析。结果普通型RCC中cyc lin D1的mRNA含量0.488±0.399,高于正常对照组0.089±0.066(P<0.01)。cyc lin D1的表达与肿瘤体积大小有关,体积大者cyc lin D1高表达(P<0.05)。普通型RCC中p27k ip1表达低于正常对照组,随着p27k ip1表达的降低,肿瘤的细胞核Fu-hrm an分级、TNM分期增高。结论cyc lin D1高表达和p27k ip1的低表达与普通型RCC的发生有关;p27k ip1的低表达可能促进肿瘤的演进,p27k ip1的表达可作为评价普通型RCC预后的参考指标。
文摘网络直播带货是近几年新兴的线上购物方式,在数据收集流通、购物支付方式以及购物体验等方面实现对传统购物的升级,同时也成为网络直播变现的另一主要途径。在当前网络平台具有虚拟性、互动性、广泛性的情况下,直播平台的法律责任认定存在直播虚拟化、功能定位不准确、以及法律依据不健全等难题,需要通过提高技术手段、建立监管制度、完善相关法律等方式明确直播带货平台的法律责任,否则将对电商行业带来难以消除的负面影响。Online live streaming sales is a new way of online shopping in recent years. It has upgraded traditional shopping in terms of data collection and circulation, shopping payment methods and shopping experience, and has also become another main way to realize online live streaming. In the current network platform with virtual, interactive, extensive, live platform of legal liability that live virtualization, function positioning is not accurate, and the legal basis is not sound, need to improve technical means, establish regulatory system, improve the relevant legal way clear live with goods platform of legal responsibility, otherwise will bring difficult to eliminate the negative impact of the electricity industry.
