自从淋巴细胞杂交瘤1975年创立以来(Kohler et al.,1975)越来越显示其优越性,(Sikora et al.,1982),从其发展看来,前景广阔,针对恶性肿瘤,白血病和组织移植等提出了崭新的疗法。由于鼠源性单克隆抗体在临床应用上往往引起过敏反应和形...自从淋巴细胞杂交瘤1975年创立以来(Kohler et al.,1975)越来越显示其优越性,(Sikora et al.,1982),从其发展看来,前景广阔,针对恶性肿瘤,白血病和组织移植等提出了崭新的疗法。由于鼠源性单克隆抗体在临床应用上往往引起过敏反应和形成免疫复合物的危险性,激励着科学家们去开拓人—人杂交瘤,尽管前进的道路上还存在着许多困难,但人们还是不屈不挠地前进。目前,世界上共有三个人—人杂交瘤系统。(1)0lsson和Kaplan等培育的SKO—007人骨髓瘤细胞系统。(2)Croce等培育的GM 1500细胞系统。(3)Clark等的RPMI8226细胞系统。并且都进行了人—人杂交瘤的研究,但是多数杂交瘤阳性率低和分泌抗体量少。国内北京、上海、西安和昆明等都正在开展本项研究。我们从1984年8月,开始人单抗的研究,也碰到许多困难,深深体会到建立一个具有我国特色的人—人杂交瘤系统是十分重要的,现将我们的工作简要报告如下。展开更多
Ⅰ. INTRODUCTION There are essentially three reasons why human monoclonal antibodies are attractive to the investigator. The first is that the specificity of oxenogenic antibodies is often poor. Secondly, human origin...Ⅰ. INTRODUCTION There are essentially three reasons why human monoclonal antibodies are attractive to the investigator. The first is that the specificity of oxenogenic antibodies is often poor. Secondly, human origin monoclonal antibodies would be more useful for dissecting human allogenic immune responses, i. e. responses to histocompatibility and blood group antigens. Thirdly, they are of interest in capturing human immune responses such as antibodies produced in lymph nodes draining a tumor or autoimmune responses, and producing of human antibodies for possible therapeutic applications.展开更多
文摘自从淋巴细胞杂交瘤1975年创立以来(Kohler et al.,1975)越来越显示其优越性,(Sikora et al.,1982),从其发展看来,前景广阔,针对恶性肿瘤,白血病和组织移植等提出了崭新的疗法。由于鼠源性单克隆抗体在临床应用上往往引起过敏反应和形成免疫复合物的危险性,激励着科学家们去开拓人—人杂交瘤,尽管前进的道路上还存在着许多困难,但人们还是不屈不挠地前进。目前,世界上共有三个人—人杂交瘤系统。(1)0lsson和Kaplan等培育的SKO—007人骨髓瘤细胞系统。(2)Croce等培育的GM 1500细胞系统。(3)Clark等的RPMI8226细胞系统。并且都进行了人—人杂交瘤的研究,但是多数杂交瘤阳性率低和分泌抗体量少。国内北京、上海、西安和昆明等都正在开展本项研究。我们从1984年8月,开始人单抗的研究,也碰到许多困难,深深体会到建立一个具有我国特色的人—人杂交瘤系统是十分重要的,现将我们的工作简要报告如下。
文摘Ⅰ. INTRODUCTION There are essentially three reasons why human monoclonal antibodies are attractive to the investigator. The first is that the specificity of oxenogenic antibodies is often poor. Secondly, human origin monoclonal antibodies would be more useful for dissecting human allogenic immune responses, i. e. responses to histocompatibility and blood group antigens. Thirdly, they are of interest in capturing human immune responses such as antibodies produced in lymph nodes draining a tumor or autoimmune responses, and producing of human antibodies for possible therapeutic applications.
