目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,...目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选苍膝通痹胶囊的活性成分及对应靶点;运用Drugbank、人类基因数据库(the human gene database,GeneCards)、药物靶标数据库(therapeutic targetdatabase,TTD)、比较毒理基因组学数据库(comparative toxicogenomics database,CTD)检索OA的已知疾病靶点;使用Venny平台得到苍膝通痹胶囊治疗OA的药效靶点。运用Cytoscape 3.7.2软件构建“药物-成分-靶点-疾病”网络,运用STRING数据库建立蛋白-蛋白互作网络(protein-protein interactions,PPI),利用CytoHubba工具对所得PPI网络进行拓扑分析筛选出关键靶点。借助DAVID数据库对作用靶点进行基因本体论功能富集分析(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)获取其潜在作用机制,构建苍膝通痹胶囊“通路-靶点”作用网络。从RCSBPDB数据库获取靶点的晶体结构文件,从Pubchem数据库获取活性成分的结构文件,利用Auto vina软件进行分子对接验证。结果:共得到苍膝通痹胶囊活性成分60个、作用靶点140个。OA疾病靶点469个,苍膝通痹胶囊治疗OA的靶点52个。核心成分有槲皮素、β-谷甾醇、豆甾醇、木犀草素等。核心靶点包括IL-1β、IL-6、VEGFA、MAPK、JUN等。通路主要涉及PI3K-Akt、NF-κB、FoxO、雌激素、MAPK通路等。有效成分在与核心靶点对接中具有较强的结合能力。结论:苍膝通痹胶囊通过调控细胞增殖、抑制炎症等多成分、多靶点、多通路治疗OA。展开更多
本文选用钢纤维和聚丙烯纤维两种常见纤维开展试验,通过测试砂浆的力学性能、粘结劈拉强度等指标,探究纤维对砂浆性能的影响规律及其作用机理。结果表明,适量掺入纤维可显著提升砂浆的综合性能。当钢纤维掺量为1.5%时,砂浆的28天抗压、...本文选用钢纤维和聚丙烯纤维两种常见纤维开展试验,通过测试砂浆的力学性能、粘结劈拉强度等指标,探究纤维对砂浆性能的影响规律及其作用机理。结果表明,适量掺入纤维可显著提升砂浆的综合性能。当钢纤维掺量为1.5%时,砂浆的28天抗压、抗折强度分别较空白组提高8.35%、87.00%,力学性能增强效果最佳。钢纤维掺量为2.0%时,砂浆粘结劈拉强度达到峰值,相比空白组提升207.84%。与钢纤维相比,聚丙烯纤维对砂浆的性能改善幅度较小,掺量为0.2%时,砂浆的抗压及抗折强度达到最大值。分析可知,纤维主要通过桥联作用和阻裂作用改善砂浆性能,但过量掺入会引起纤维团聚,导致砂浆基体缺陷增多、性能降低。此外,钢纤维可增大砂浆–混凝土界面摩擦阻力和机械咬合力,从而提高砂浆粘结性能。In this paper, two common fibers, steel fiber and polypropylene fiber, are selected to carry out experiments. By testing the mechanical properties of mortar, bond splitting strength and other indicators, the influence of fiber on the performance of mortar and its mechanism are explored. The results show that the appropriate amount of fiber can significantly improve the comprehensive properties of mortar. When the steel fiber content is 1.5%, the 28-day compressive strength and flexural strength of mortar are increased by 8.35% and 87.00%, respectively, compared with the blank group, and the mechanical properties are enhanced best. When the steel fiber content is 2.0%, the adhesive splitting tensile strength of mortar reaches the peak value, which is 207.84% higher than that of blank group. Compared with steel fiber, polypropylene fiber can improve the performance of mortar less. When the content is 0.2%, the compressive strength and flexural strength of mortar reach the maximum. The analysis shows that fiber can improve the performance of mortar mainly through bridging and crack inhibition, but excessive incorporation will cause fiber agglomeration, resulting in increased defects and reduced performance of mortar matrix. In addition, steel fiber can increase the friction resistance and mechanical bite force of mortar - concrete interface, so as to improve the bond property of mortar.展开更多
文摘目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选苍膝通痹胶囊的活性成分及对应靶点;运用Drugbank、人类基因数据库(the human gene database,GeneCards)、药物靶标数据库(therapeutic targetdatabase,TTD)、比较毒理基因组学数据库(comparative toxicogenomics database,CTD)检索OA的已知疾病靶点;使用Venny平台得到苍膝通痹胶囊治疗OA的药效靶点。运用Cytoscape 3.7.2软件构建“药物-成分-靶点-疾病”网络,运用STRING数据库建立蛋白-蛋白互作网络(protein-protein interactions,PPI),利用CytoHubba工具对所得PPI网络进行拓扑分析筛选出关键靶点。借助DAVID数据库对作用靶点进行基因本体论功能富集分析(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)获取其潜在作用机制,构建苍膝通痹胶囊“通路-靶点”作用网络。从RCSBPDB数据库获取靶点的晶体结构文件,从Pubchem数据库获取活性成分的结构文件,利用Auto vina软件进行分子对接验证。结果:共得到苍膝通痹胶囊活性成分60个、作用靶点140个。OA疾病靶点469个,苍膝通痹胶囊治疗OA的靶点52个。核心成分有槲皮素、β-谷甾醇、豆甾醇、木犀草素等。核心靶点包括IL-1β、IL-6、VEGFA、MAPK、JUN等。通路主要涉及PI3K-Akt、NF-κB、FoxO、雌激素、MAPK通路等。有效成分在与核心靶点对接中具有较强的结合能力。结论:苍膝通痹胶囊通过调控细胞增殖、抑制炎症等多成分、多靶点、多通路治疗OA。
文摘本文选用钢纤维和聚丙烯纤维两种常见纤维开展试验,通过测试砂浆的力学性能、粘结劈拉强度等指标,探究纤维对砂浆性能的影响规律及其作用机理。结果表明,适量掺入纤维可显著提升砂浆的综合性能。当钢纤维掺量为1.5%时,砂浆的28天抗压、抗折强度分别较空白组提高8.35%、87.00%,力学性能增强效果最佳。钢纤维掺量为2.0%时,砂浆粘结劈拉强度达到峰值,相比空白组提升207.84%。与钢纤维相比,聚丙烯纤维对砂浆的性能改善幅度较小,掺量为0.2%时,砂浆的抗压及抗折强度达到最大值。分析可知,纤维主要通过桥联作用和阻裂作用改善砂浆性能,但过量掺入会引起纤维团聚,导致砂浆基体缺陷增多、性能降低。此外,钢纤维可增大砂浆–混凝土界面摩擦阻力和机械咬合力,从而提高砂浆粘结性能。In this paper, two common fibers, steel fiber and polypropylene fiber, are selected to carry out experiments. By testing the mechanical properties of mortar, bond splitting strength and other indicators, the influence of fiber on the performance of mortar and its mechanism are explored. The results show that the appropriate amount of fiber can significantly improve the comprehensive properties of mortar. When the steel fiber content is 1.5%, the 28-day compressive strength and flexural strength of mortar are increased by 8.35% and 87.00%, respectively, compared with the blank group, and the mechanical properties are enhanced best. When the steel fiber content is 2.0%, the adhesive splitting tensile strength of mortar reaches the peak value, which is 207.84% higher than that of blank group. Compared with steel fiber, polypropylene fiber can improve the performance of mortar less. When the content is 0.2%, the compressive strength and flexural strength of mortar reach the maximum. The analysis shows that fiber can improve the performance of mortar mainly through bridging and crack inhibition, but excessive incorporation will cause fiber agglomeration, resulting in increased defects and reduced performance of mortar matrix. In addition, steel fiber can increase the friction resistance and mechanical bite force of mortar - concrete interface, so as to improve the bond property of mortar.
