目的探讨肾上腺髓质素(adrenom edu llin,ADM)在不同月龄大鼠脑缺血再灌注(I/R)脑组织的表达情况。方法采用栓线法制成大鼠大脑中动脉I/R模型,阻断血流2 h后再灌注4 h。应用免疫组织化学染色法检测不同月龄段大鼠局灶性脑I/R后血浆ADM...目的探讨肾上腺髓质素(adrenom edu llin,ADM)在不同月龄大鼠脑缺血再灌注(I/R)脑组织的表达情况。方法采用栓线法制成大鼠大脑中动脉I/R模型,阻断血流2 h后再灌注4 h。应用免疫组织化学染色法检测不同月龄段大鼠局灶性脑I/R后血浆ADM。结果正常大鼠脑内即有ADM表达,假手术后ADM表达略有增加,但与正常对照组相比无明显差异(P>0.05);大鼠脑I/R后ADM免疫阳性细胞增多,与正常对照组及假手术组相比差异显著(均P<0.05);大鼠脑I/R后缺血侧及缺血对侧ADM免疫阳性细胞均增多,但以缺血侧区域增多最为明显(P<0.05)。老龄大鼠脑I/R后ADM免疫阳性细胞数明显高于青年大鼠(P<0.05)。结论脑I/R后ADM表达增强,ADM表达增强与血管硬化相关。展开更多
Objective To observe the influence of adrenomedullin （ADM） on neuron apoptosis, infarction volume of brain, and the expression of early growth response 1 （Egr-1） mRNA in ischemia-reperfusion rats. Methods The arte...Objective To observe the influence of adrenomedullin （ADM） on neuron apoptosis, infarction volume of brain, and the expression of early growth response 1 （Egr-1） mRNA in ischemia-reperfusion rats. Methods The arteria cerebri media was tied for 2 h to construct the ischemia model. Infarction volume was detected by triphenltetrazolium chloride （T＇I＇C） staining, neuronal apoptosis and necrosis was detected with terminal deoxynucleotidyl transferase nick labeling （TUNEL） method, and the Egr-1 mRNA expression was examined by in situ hybridization （ISH）. Results Infarction volume after ischemia-reperfusion is （269 ± 20） mm^3. Infarction volume after injection of ADM through different ways are femoral vein （239 ± 17） mm^3 （decreased by 11.2%）, arteria carotis （214 ± 14） mm^3 （by 20.4%） and lateral cerebral ventricle （209 ± 13） mm^3 （by 22.3%）, respectively. The results indicate that injecting ADM through arteria carotis and lateral cerebral ventricle is much more effective than it through femoral vein （P 〈 0.05）. The TUNEL-positive cells in cerebral cortex or hippocampus are few in the sham operation group, but much more in the ischemia-reperfusion group. After being supplied with ADM, especially through arteria carotis interna or lateral cerebral ventricle way, the TUNEL-positive cells decreased obviously. Expression of Egr- 1 mRNA was low in the cerebral cortex of the sham operation group rats, enhanced in the ischemia and reperfusion group rats, and enhanced markedly after treatment with ADM, especially through arteria carotis interna or lateral cerebral ventricle way （P 〈 0.01）. Conclusion Injection of ADM through different ways could alleviate neural dysfunction, decrease neuron apoptosis and brain infarction volume, and increase the expression of Egr- 1 mRNA.展开更多
文摘目的探讨肾上腺髓质素(adrenom edu llin,ADM)在不同月龄大鼠脑缺血再灌注(I/R)脑组织的表达情况。方法采用栓线法制成大鼠大脑中动脉I/R模型,阻断血流2 h后再灌注4 h。应用免疫组织化学染色法检测不同月龄段大鼠局灶性脑I/R后血浆ADM。结果正常大鼠脑内即有ADM表达,假手术后ADM表达略有增加,但与正常对照组相比无明显差异(P>0.05);大鼠脑I/R后ADM免疫阳性细胞增多,与正常对照组及假手术组相比差异显著(均P<0.05);大鼠脑I/R后缺血侧及缺血对侧ADM免疫阳性细胞均增多,但以缺血侧区域增多最为明显(P<0.05)。老龄大鼠脑I/R后ADM免疫阳性细胞数明显高于青年大鼠(P<0.05)。结论脑I/R后ADM表达增强,ADM表达增强与血管硬化相关。
文摘Objective To observe the influence of adrenomedullin （ADM） on neuron apoptosis, infarction volume of brain, and the expression of early growth response 1 （Egr-1） mRNA in ischemia-reperfusion rats. Methods The arteria cerebri media was tied for 2 h to construct the ischemia model. Infarction volume was detected by triphenltetrazolium chloride （T＇I＇C） staining, neuronal apoptosis and necrosis was detected with terminal deoxynucleotidyl transferase nick labeling （TUNEL） method, and the Egr-1 mRNA expression was examined by in situ hybridization （ISH）. Results Infarction volume after ischemia-reperfusion is （269 ± 20） mm^3. Infarction volume after injection of ADM through different ways are femoral vein （239 ± 17） mm^3 （decreased by 11.2%）, arteria carotis （214 ± 14） mm^3 （by 20.4%） and lateral cerebral ventricle （209 ± 13） mm^3 （by 22.3%）, respectively. The results indicate that injecting ADM through arteria carotis and lateral cerebral ventricle is much more effective than it through femoral vein （P 〈 0.05）. The TUNEL-positive cells in cerebral cortex or hippocampus are few in the sham operation group, but much more in the ischemia-reperfusion group. After being supplied with ADM, especially through arteria carotis interna or lateral cerebral ventricle way, the TUNEL-positive cells decreased obviously. Expression of Egr- 1 mRNA was low in the cerebral cortex of the sham operation group rats, enhanced in the ischemia and reperfusion group rats, and enhanced markedly after treatment with ADM, especially through arteria carotis interna or lateral cerebral ventricle way （P 〈 0.01）. Conclusion Injection of ADM through different ways could alleviate neural dysfunction, decrease neuron apoptosis and brain infarction volume, and increase the expression of Egr- 1 mRNA.
