Pepsinogens are zymogens of pepsins,aspecific proteases working as digestive enzymes in vertebrate stomach,of which biological and molecular properties have been extensively studied.Several exhaustive studies have bee...Pepsinogens are zymogens of pepsins,aspecific proteases working as digestive enzymes in vertebrate stomach,of which biological and molecular properties have been extensively studied.Several exhaustive studies have been performed in the pepsinogen producing cells in developing rat stomachs,but little is known about the expression of pepsinogen gene in these cells.In this study,the ontogeny of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization using a digoxigenin-labeled RNA probe.The rat gastric epithelium was stratified but was morphologically undifferentiated at the stage of 18.5 days of gestation.The pepsinogen mRNA was expressed both in chief cells and mucous neck cells in adult rats,which was first detected by in situ hybridization in the stomach of the rats at 3.5 days after birth.The development of pepsinogen producing cells could be classified into four stages:(1) 18.5 days of gestation to 0.5 day after birth;(2) 3.5 days to 2 weeks after birth;(3) 3～4 weeks after birth;(4) 8 weeks after birth.Pepsinogen expression is strictly limited to these cells,the distribution of which shown a developmental stage-specific manner.We concluded the pepsinogen C could offer excellent molecular markers of differentiation during stomach epithelial cellulur development.展开更多
目的:研究缺氧对人视网膜色素上皮细胞-19(ARPE-19)的增生和血管内皮细胞生长因子表达的诱导作用。方法:实验于2004-10/2005-12于南京医科大学生理学实验室完成。实验材料:ARPE-19细胞购自美国American Type Culture Collection。实验...目的:研究缺氧对人视网膜色素上皮细胞-19(ARPE-19)的增生和血管内皮细胞生长因子表达的诱导作用。方法:实验于2004-10/2005-12于南京医科大学生理学实验室完成。实验材料:ARPE-19细胞购自美国American Type Culture Collection。实验方法及分组:ARPE-19细胞被暴露在含体积分数为0.05的CO2和0.95的N2的缺氧培养缸中培养为缺氧组。同代同一培养瓶中传代的细胞在常氧情况下培养,作为正常对照组。实验评估:2,12,24,36h后,光镜下观察ARPE-19细胞形态学变化,采用四甲基偶氮唑盐法检测两组ARPE-19细胞增殖的情况;用半定量反转录聚合酶链反应检测两组ARPE-19细胞血管内皮细胞生长因子mRNA的表达。结果:①缺氧对ARPE-19细胞增殖的影响:ARPE-19细胞缺氧组2,12,24,36hA值均比正常组明显增高,除缺氧2h外,其他各时间点均能明显诱导ARPE-19细胞增殖(P<0.05)。②缺氧对血管内皮生长因子mRNA表达的影响:缺氧组血管内皮生长因子mRNA表达明显增高,缺氧2,12,24,36h血管内皮生长因子mRNA表达均高于正常对照组(1.567±0.080,1.868±0.176,5.061±0.407,1.748±0.277,0.603±0.017,P<0.05,n=8),并且呈时间依赖性,在24h达到表达最高峰。结论:缺氧可以促进ARPE-19细胞增殖和血管内皮生长因子mRNA表达增加,为研究缺血缺氧性视网膜疾病提供了一个新的思路,即控制缺氧造成的视网膜色素上皮细胞增殖和血管内皮细胞增殖对治疗缺血缺氧性视网膜疾病是绝对必要的。展开更多
文摘Pepsinogens are zymogens of pepsins,aspecific proteases working as digestive enzymes in vertebrate stomach,of which biological and molecular properties have been extensively studied.Several exhaustive studies have been performed in the pepsinogen producing cells in developing rat stomachs,but little is known about the expression of pepsinogen gene in these cells.In this study,the ontogeny of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization using a digoxigenin-labeled RNA probe.The rat gastric epithelium was stratified but was morphologically undifferentiated at the stage of 18.5 days of gestation.The pepsinogen mRNA was expressed both in chief cells and mucous neck cells in adult rats,which was first detected by in situ hybridization in the stomach of the rats at 3.5 days after birth.The development of pepsinogen producing cells could be classified into four stages:(1) 18.5 days of gestation to 0.5 day after birth;(2) 3.5 days to 2 weeks after birth;(3) 3～4 weeks after birth;(4) 8 weeks after birth.Pepsinogen expression is strictly limited to these cells,the distribution of which shown a developmental stage-specific manner.We concluded the pepsinogen C could offer excellent molecular markers of differentiation during stomach epithelial cellulur development.
文摘目的:研究缺氧对人视网膜色素上皮细胞-19(ARPE-19)的增生和血管内皮细胞生长因子表达的诱导作用。方法:实验于2004-10/2005-12于南京医科大学生理学实验室完成。实验材料:ARPE-19细胞购自美国American Type Culture Collection。实验方法及分组:ARPE-19细胞被暴露在含体积分数为0.05的CO2和0.95的N2的缺氧培养缸中培养为缺氧组。同代同一培养瓶中传代的细胞在常氧情况下培养,作为正常对照组。实验评估:2,12,24,36h后,光镜下观察ARPE-19细胞形态学变化,采用四甲基偶氮唑盐法检测两组ARPE-19细胞增殖的情况;用半定量反转录聚合酶链反应检测两组ARPE-19细胞血管内皮细胞生长因子mRNA的表达。结果:①缺氧对ARPE-19细胞增殖的影响:ARPE-19细胞缺氧组2,12,24,36hA值均比正常组明显增高,除缺氧2h外,其他各时间点均能明显诱导ARPE-19细胞增殖(P<0.05)。②缺氧对血管内皮生长因子mRNA表达的影响:缺氧组血管内皮生长因子mRNA表达明显增高,缺氧2,12,24,36h血管内皮生长因子mRNA表达均高于正常对照组(1.567±0.080,1.868±0.176,5.061±0.407,1.748±0.277,0.603±0.017,P<0.05,n=8),并且呈时间依赖性,在24h达到表达最高峰。结论:缺氧可以促进ARPE-19细胞增殖和血管内皮生长因子mRNA表达增加,为研究缺血缺氧性视网膜疾病提供了一个新的思路,即控制缺氧造成的视网膜色素上皮细胞增殖和血管内皮细胞增殖对治疗缺血缺氧性视网膜疾病是绝对必要的。
