Oil contaminated soil was collected from Huangpu River-Yangtze River estuary wetland, with the aim of isolating oil-degrading microorganisms and evaluating their ability to degrade diesel. Three bacterial strains were...Oil contaminated soil was collected from Huangpu River-Yangtze River estuary wetland, with the aim of isolating oil-degrading microorganisms and evaluating their ability to degrade diesel. Three bacterial strains were discovered and identified by sequencing their 16S rDNA genes, two were Pseudomonas and one was Alcaligcnes. The proper growth conditions of each bacterium were measured and presented for diesel biodegradation. Biodegradation assays revealed that the degradation rates of three bacterial strains were 42.5%, 14.6% and 15.9% in 7 d respectively. They all play an important role on the nalkanes within the range of C16-C25 components of diesel. The results indicated that the oil-degraders can adapt to degrade diesel. The bacterial strains can be used in wetland diesel pollution control.展开更多
Oil spills may considerably damage sensitive coastal wetlands. The phytoremediation potential and restoration of a dominant coastal marsh plant, Cyperus rotundus, for diesel pollutant and its phytoremediation effectiv...Oil spills may considerably damage sensitive coastal wetlands. The phytoremediation potential and restoration of a dominant coastal marsh plant, Cyperus rotundus, for diesel pollutant and its phytoremediation effectiveness were investigated in this open-air pot experiment. Cyperus rotundus was transplanted into soil contaminated with diesel at concentrations of 1 000, 5 000, 10 000, 15 000, 20 000 mg/kg. In order to better elucidate the biochemical and physiological responses to diesel pollutants, activity of the antioxidant enzymes peroxidase （POD）, catalase （CAT） and ascorbic acid oxidase （AAO） were determined in the plant tissue after 50 d treatment at the levels mentioned above. The results showed that CAT and AAO of stem and leaf exhibited peak enzyme activities on 15 000 mg/kg soil and 10 000 mg/kg soil respectively, and declined at higher concentrations. Additionally, the increment of biomass and the content of soluble protein, as well as chlorophyll content were affected by diesel. The highest restoration effectiveness appeared at the level of 5 000 mg/kg. Collectively, Cyperus rotundus is a potential plant which can be used for restoring the diesel-contaminated soil.展开更多
Daqing Wetland was contaminated by heavy metals for holding lots of industrial and domestic waste water. Due to their high stability and non-degradation, heavy metals are possible to gather in human body through the f...Daqing Wetland was contaminated by heavy metals for holding lots of industrial and domestic waste water. Due to their high stability and non-degradation, heavy metals are possible to gather in human body through the food chain and then threaten health. Water samples were collected from Longfeng Wetland and lake marshes in the vicinity of Daqing City. The contents of heavy metals （Cd, Pb, Cr, Cu, Zn, Ni） were determined, and the contamination degrees were evaluated simultaneously. The results indicate that heavy metals of Zn, Ni, Cd, Cr and Pd have contaminated the Longfeng Wetland severely. In addition the concentrations of Cr and Cd in some lakes around oil wells are excessively high, and have overrun the grade V based on the threshold values of surface water given by the National Environmental Protection Agency of China. Some measures have to be taken to tackle heavy metal contamination of Longfeng Wetland and lake marshes in Daqing City.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant Nos.40973073,40830744)the Shanghai Leading Academic Discipline Project(Grant No.S30109)the Innovation Foundation of Shanghai University
文摘Oil contaminated soil was collected from Huangpu River-Yangtze River estuary wetland, with the aim of isolating oil-degrading microorganisms and evaluating their ability to degrade diesel. Three bacterial strains were discovered and identified by sequencing their 16S rDNA genes, two were Pseudomonas and one was Alcaligcnes. The proper growth conditions of each bacterium were measured and presented for diesel biodegradation. Biodegradation assays revealed that the degradation rates of three bacterial strains were 42.5%, 14.6% and 15.9% in 7 d respectively. They all play an important role on the nalkanes within the range of C16-C25 components of diesel. The results indicated that the oil-degraders can adapt to degrade diesel. The bacterial strains can be used in wetland diesel pollution control.
基金Project supported by the National Natural Science Foundation of China (Grant Nos.40973073, 41073072)the Shanghai Leading Academic Discipline Project (Grant No.S30109),and the Technology Foundation of Shanghai University
文摘Oil spills may considerably damage sensitive coastal wetlands. The phytoremediation potential and restoration of a dominant coastal marsh plant, Cyperus rotundus, for diesel pollutant and its phytoremediation effectiveness were investigated in this open-air pot experiment. Cyperus rotundus was transplanted into soil contaminated with diesel at concentrations of 1 000, 5 000, 10 000, 15 000, 20 000 mg/kg. In order to better elucidate the biochemical and physiological responses to diesel pollutants, activity of the antioxidant enzymes peroxidase （POD）, catalase （CAT） and ascorbic acid oxidase （AAO） were determined in the plant tissue after 50 d treatment at the levels mentioned above. The results showed that CAT and AAO of stem and leaf exhibited peak enzyme activities on 15 000 mg/kg soil and 10 000 mg/kg soil respectively, and declined at higher concentrations. Additionally, the increment of biomass and the content of soluble protein, as well as chlorophyll content were affected by diesel. The highest restoration effectiveness appeared at the level of 5 000 mg/kg. Collectively, Cyperus rotundus is a potential plant which can be used for restoring the diesel-contaminated soil.
基金Project supported by the Shanghai Leading Academic Discipline Project(Grant No.S30109)the Innovation Foundation of Shanghai University
文摘Daqing Wetland was contaminated by heavy metals for holding lots of industrial and domestic waste water. Due to their high stability and non-degradation, heavy metals are possible to gather in human body through the food chain and then threaten health. Water samples were collected from Longfeng Wetland and lake marshes in the vicinity of Daqing City. The contents of heavy metals （Cd, Pb, Cr, Cu, Zn, Ni） were determined, and the contamination degrees were evaluated simultaneously. The results indicate that heavy metals of Zn, Ni, Cd, Cr and Pd have contaminated the Longfeng Wetland severely. In addition the concentrations of Cr and Cd in some lakes around oil wells are excessively high, and have overrun the grade V based on the threshold values of surface water given by the National Environmental Protection Agency of China. Some measures have to be taken to tackle heavy metal contamination of Longfeng Wetland and lake marshes in Daqing City.
